BD FACSCanto - HTS Hardware and Components Overview

14 BD High Throughput Sampler User’s Guide
HTS Hardware Overview
Easy to use and maintain, and highly reliable, the HTS provides the following
basic functionality:
• Acquires samples from 96- and 384-well plates (standard depth)
• Includes two throughput modes: standard and high throughput
• Minimizes carryover
• Provides user-definable mixing and sample introduction protocols
• Is user-installable (software and sampler unit only; initial installation
excluded)
• Supports immunophenotyping assays
The HTS can process a 96-well plate in approximately 44 minutes in standard
mode and approximately 15 minutes in high-throughput mode using the default
settings listed in Table 1-1.
Table 1-1 Default throughput mode settings
Setting
Standard
Mode
High-Throughput
Mode
Sample Flow Rate (µL/sec) 1 1
Sample Volume (µL) 10 2
Mixing Volume (µL) 100 50
Mixing Speed (µL/sec) 180 200
Number of Mixes (cycles) 2 2
Wash Volume (µL) 400 200
Approximate Acquisition Time (min) 44 15