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BD FACSCanto - Decontaminating the Fluidics Manually

BD FACSCanto
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Appendix B: Depot Repair Procedures 163
Use a 10% bleach solution to decontaminate the internal fluidics and external
surfaces of the HTS unit.
1 Pour 45 mL of DI water into a 100-mL beaker.
2 Carefully add 5 mL of undiluted bleach to the same beaker.
3 Gently swirl the solution to mix.
Decontaminating the Fluidics Manually
Materials Needed
household bleach
DI water
disposable plastic pipettes
line declogger, PN 343583 from the accessories kit (for manual fluidics
decontamination only)
Cleaning the Fluidics
Perform this procedure to decontaminate the internal fluidics of a non-functional
HTS unit.
1 Remove the sample probe and tubing that connects the probe to the
primary pump; discard the probe and tubing (Figure B-3 on page 164).
To prevent shock, verify that the cytometer is turned off and the power
cable is detached before you start cleaning.
To ensure that the 10% bleach solution retains its full germicidal effect,
prepare a fresh solution daily.

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