Chapter 4: Maintenance 121
7 Install the new filter.
• Attach the tubing segment with the male connector to the female filter
connection. Hold the filter while you twist the luer lock ring.
• Attach the tubing segment with the female connector to the male filter
connection. Hold the filter while you twist the luer female connector.
8 Reinstall the sheath line between the cytometer interface panel and the
HTS.
Attach the quick-connectors at each end.
NOTICE Although the sheath filter is a screen filter that provides identical
performance regardless of the flow direction, BD recommends that you
always install the sheath line with the lock-ring end toward the cytometer
panel (connector labeled C). This will prevent any foreign material
accumulated on one side of the filter from flushing into the HTS if the filter
is reversed.
9 Reinstall the HTS unit.
- [BD FACSCanto] Install the rear catch tray by routing the sample
coupler underneath the catch tray tongue and sample coupler slot.
Push the tray in, making sure the coupler line is not crimped or kinked.
- Push in the unit and tighten the positioning screw holding the HTS to
the cytometer support bracket.
Cleaning the Air Filter
The air filter element is located within a filter retainer and fan guard attached to
the base plate of the HTS unit. To clean or replace the air filter, follow these
steps.
1 Remove the HTS unit from the cytometer.
For detailed instructions, see Removing the HTS Unit on page 159.