132 BD High Throughput Sampler User’s Guide
6 Install a new O-ring and slide it to about the middle of the tube.
O-rings are included in the accessory kit.
7 Slide the SIT protector straight onto the SIT.
8 While holding the tube, gently push up on the retainer screw and screw it
on to secure it.
9 Reattach the sample coupler to the cytometer SIT.
Slide the sample coupler onto the SIT until you reach a hard stop. Make
sure the sample coupler tubing is not kinked or twisted. Hold the coupler
with one hand while you tighten the top nut with the other hand.
Note that there should be a gap between the tightening nut and the bottom
of the SIT protector. If you don’t see a gap, unscrew the tube retainer, push
the SIT protector all the way up, and retighten the tube retainer.
Placing HTS Unit into Long-Term Storage
To prevent salt deposits from forming in the fluidics system, perform the
following procedure if you will not be using your HTS unit for a week or more.
See Cleaning the HTS Unit on page 162.
Placing the HTS for BD LSR II in Long-Term Storage
1 Remove the HTS safety cover.
2 Locate and loosen the positioning screw that secures the HTS to the
cytometer support bracket (Figure 4-17 on page 133).
To avoid bending the SIT, do not slide the SIT protector at an angle.
All cytometer surfaces that come in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
cytometer components. Wear suitable protective clothing, eyewear, and gloves.
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