100 BD High Throughput Sampler User’s Guide
NOTICE If you exchanged BD FACSFlow with BD FACS sheath solution with
surfactant, install the HTS sample coupler, then prime the HTS twice by choosing
HTS > Prime. This will exchange the fluid in the syringes with BD FACS sheath
solution with surfactant.
Shutting Down
Perform a daily cleaning procedure before shutting down the system.
1 Run a daily cleaning procedure (see Daily Cleaning on page 104).
2 [BD FACSCanto and BD FACSCanto II] Perform a fluidics shutdown. Refer
to Fluidics Shutdown in the following section.
3 [BD LSR II] Prime the HTS unit.
- Place the cytometer in Run mode.
- Choose HTS > Prime.
- When priming is complete, click OK in the dialog, then place the
cytometer in Standby mode.
NOTICE To prime the cytometer, press the Prime button on the fluid
control panel on the cytometer itself.
4 [BD FACSCanto and BD LSR II] Turn off the power to the HTS.
5 Turn off the power to the cytometer.
6 Quit the software (see Quitting the Software on page 101).
Fluidics Shutdown
[BD FACSCanto and BD FACSCanto II] Perform a fluidics shutdown before
quitting the software.
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