BD FACSCanto - Setting up the Worksheet

74 BD High Throughput Sampler User’s Guide
Setting Up the Worksheet
This section describes how to set up plots in a global worksheet.
1 Create an FSC vs SSC plot on the global worksheet.
The plot parameters should be FSC-A and SSC-A.
2 Create a PE histogram next to the FSC vs SSC dot plot; change the plot
parameter to PE-A.
3 Create the following dot plots choosing the appropriate parameters.
• FITC vs PE
• PerCP-Cy5.5 vs PE-Cy7
• APC vs APC-Cy7
4 Show a population hierarchy.
The worksheet should look similar to Figure 3-12 on page 75.