56 BD High Throughput Sampler User’s Guide
Starting Up
This section describes how to start up the cytometer and software. Notice that
some steps apply only to a specific cytometer(s).
1 Start up the flow cytometer as described in the appropriate cytometer
manual.
Make sure to refill the sheath container and empty the waste container. If a
full waste container is detected at the start of or during a run, the run will
be stopped. If necessary, perform a sheath fluid exchange to use the
appropriate sheath solution. See Exchanging the Sheath Fluid on a
BD LSR II on page 96 or Exchanging the Sheath Fluid on a BD FACSCanto
and BD FACSCanto II on page 98.
2 Turn on the HTS.
NOTICE The HTS turns on automatically for the BD FACSCanto II.
3 Ensure the sample coupler is installed. See details under Setting Up for
Plate-Based Acquisition on page 59.
To prevent bubble formation in the flow cell, use only BD FACS sheath
solution with surfactant (BD Catalog No. 336524 [US] or 336911
[Europe]) when acquiring samples with the HTS. This sheath solution is
intended for research use only, and should be used only with the HTS
option. It should not be used for sorting or in vitro diagnostic (IVD)
applications. Refer to the product insert for more information.
Add 500 µL of Sigma® Antifoam A Concentrate to the waste tank to
prevent foaming of potentially biohazardous waste up around the cap. Mix
the Antifoam Concentrate in distilled water to force it into solution before
adding it to the waste tank. Add the Antifoam Concentrate in addition to
bleach.
Do not allow the system to run dry, as this could damage the HTS pumps.
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