BD FACSCanto - Returning to Tube-Based Acquisition on the BD Facscanto

Chapter 3: Running Samples 97
Returning to Tube-Based Acquisition on the
BD FACSCanto and BD FACSCanto II
Before running BD FACSCanto clinical software, you must exchange BD FACS
sheath solution with surfactant with BD FACSFlow. Version 2.1 of the clinical
software will not connect in acquisition mode if the sheath type is set to
BD FACS sheath solution with surfactant.
1 Perform a sheath exchange to replace BD FACS sheath solution with
surfactant with BD FACSFlow. See Exchanging the Sheath Fluid on a
BD FACSCanto and BD FACSCanto II on page 98.
2 Detach the sample coupler from the cytometer SIT by unscrewing the top
thumbscrew.
Once the coupler feels free, gently pull it straight down from the SIT.
NOTICE For the BD FACSCanto, place the sample coupler in the clamp on
the catch tray when the coupler is not in use (see Figure B-11 on page 173).
To avoid bending the SIT, pull straight down on the coupler. Do not pull the
coupler at an angle.