BD FACSCanto - Returning to Tube-Based Acquisition; Returning to Tube-Based Acquisition on the BD LSR II

94 BD High Throughput Sampler User’s Guide
Returning to Tube-Based Acquisition
Returning to Tube-Based Acquisition on the BD LSR II
To return to tube mode, you need to remove the sample coupler and the SIT
protector, reinstall the DCM sleeve, place the acquisition control switch in tube
mode, and remove the HTS as described in this section.
1 Place the cytometer in Standby.
2 Switch off the HTS power.
3 Detach the sample coupler from the cytometer SIT by unscrewing the top
thumbscrew.
Once the coupler feels free, gently pull it straight down from the SIT.
4 Remove the SIT protector.
Unscrew the tube retainer and slide the SIT protector straight down.
5 Reinstall the standard DCM sleeve (Figure 3-19 on page 95).
Slide the sleeve straight up over the SIT, and screw the tube retainer on to
secure it.
Any cytometer surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
cytometer hardware. Wear suitable protective clothing and gloves.
To avoid bending the SIT, pull straight down on the coupler. Do not pull the
coupler at an angle.
To avoid bending the SIT, do not slide the SIT protector at an angle.