Chapter 4: Maintenance 143
Inspecting Hexagonal Fittings
Hexagonal male fittings are located at the following locations. Check and tighten
these fittings periodically, and replace them when needed.
• Left port of primary pump
• Top, left, and right ports of secondary pump
NOTICE Valves contain sealing washers in each port. Over-tightening fittings
can compress the seal washers resulting in a blocked port. Insert a fitting or
syringe into a port until it contacts the seal washer, then turn the fitting or syringe
an additional 1/6 to 1/4 turn. If leakage is observed, tighten a maximum of an
additional 1/8 turn.
Declogging the SIT
Perform this procedure to dislodge a clog in the SIT only when instructed to do
so by BD Biosciences.
Materials
cleaning stylus
All cytometer surfaces that come in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
cytometer components. Wear suitable protective clothing, eyewear, and gloves.
All cytometer surfaces that come in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
cytometer components. Wear suitable protective clothing, eyewear, and
gloves.
To avoid a service call, do not insert the cleaning stylus all the way into the
SIT; allow at least an inch of the stylus to protrude from the end of the SIT
while declogging.