Chapter 4: Maintenance 125
Replacing a Pump Syringe
Glass syringes are used in the primary and secondary pumps in the HTS unit.
Replace a syringe once a year or whenever leaks occur or pumping accuracy is
suspect. To begin this procedure, the cytometer and software must be running.
1 Before replacing the pump syringe, perform monthly cleaning.
See Monthly Cleaning on page 111.
2 [BD LSR II] Place the cytometer in Run mode.
3 Remove the liquid from the syringe by performing steps 2 through 11 in
Placing HTS Unit into Long-Term Storage on page 132.
4 Turn off the HTS power switch (BD LSR II and BD FACSCanto), or turn
off the cytometer (BD FACSCanto II). Keep the software running on the
workstation.
5 If you are replacing:
• the primary pump syringe, located at the front of the HTS, skip to
step 7.
• the secondary pump syringe, located at the back of the HTS, remove
the HTS by following steps 1 through 13 in Removing the HTS Unit on
page 159.
All cytometer surfaces and hardware that come in contact with biological
specimens can transmit potentially fatal disease. Use universal precautions
when changing the syringe. Wear suitable protective clothing, eyewear, and
gloves.
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