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BD FACSCanto - Page 124

BD FACSCanto
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124 BD High Throughput Sampler User’s Guide
1 Remove the HTS unit from the cytometer.
For detailed instructions, see Removing the HTS Unit on page 159.
2 Detach the sample coupler tubing.
Unscrew the tubing fitting from the secondary pump valve (Figure 4-10)
and discard the sample coupler and tubing as biohazardous waste.
Figure 4-10 Detaching the sample coupler and tubing
3 Install a new sample coupler and tubing.
Replacement tubing is included in the accessory kit. Connect the new
tubing fitting to the port in the secondary pump valve. Tighten the tubing
fitting until it is finger-tight.
4 Reinstall the HTS unit on the cytometer.
For detailed instructions, see Installing the HTS Unit on page 170.
Valves contain sealing washers in each port. If you over-tighten the fitting,
the sealing washers can be compressed resulting in a blocked port. Tighten
the fitting until it contacts the sealing washer, and then turn the fitting an
additional 1/6 to 1/4 turn. If leaking is observed, tighten the fitting no more
than an additional 1/8 turn.

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