Chapter 3: Running Samples 59
8 [BD LSR II] Place the cytometer in Run mode, then choose HTS > Prime to
prime the HTS unit. When the priming is complete, click OK in the dialog.
NOTICE To prime the cytometer, press the Prime button on the fluid
control panel on the cytometer itself.
Setting Up for Plate-Based Acquisition
This section describes how to set up the cytometer for plate-based acquisition.
Setting Up for Plate-Based Acquisition on the BD FACSCanto and
BD FACSCanto II
When switching from tube to plate mode, perform a sheath fluid exchange, then
attach the sample coupler to the SIT.
1 Turn on the cytometer and the HTS.
For the BD FACSCanto, turn on the cytometer, followed by the HTS. For
the BD FACSCanto II, the HTS turns on automatically when the cytometer
is powered on.
2 Remove/open the HTS safety cover.
[BD FACSCanto] Tilt the front of the cover up, then slide the cover forward
to remove it.
[BD FACSCanto II] Slide open the covers. Slide the front cover to the left;
slide the side cover to the back.
3 Perform a sheath fluid exchange.
Refer to Exchanging the Sheath Fluid on a BD FACSCanto and
BD FACSCanto II on page 98.
Any cytometer surface that comes in contact with biological specimens can
transmit potentially fatal disease. Use universal precautions when handling
cytometer hardware. Wear suitable protective clothing and gloves.
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