146 BD High Throughput Sampler User’s Guide
HTS Troubleshooting
Observation Possible Causes Recommended Solutions
Bubbles in sample wells Mixing volume too great Reduce the mixing volume. See
Understanding Volumes on
page 25 and Mixing on page 26
for recommendations.
Too many mixes Reduce the number of mixes.
Insufficient sample volume Increase sample volume.
Leaking around sample
coupler or cytometer SIT
Coupler or tube retainer loose
or worn
Check the fittings and tighten
them as needed.
Leaking around pump
syringes
Fitting loose Tighten the fitting, and replace it
if necessary. See Inspecting
Thumbscrew Fittings on
page 142.
[BD LSR II] DCM
system running when
tube arm to side
Acquisition switch in Tube
mode
Switch to Plate mode.
Bubbles in waste tank No antifoam concentrate
being used
Add 500 µL Sigma Antifoam A
Concentrate to the waste tank.
Fluid accumulating
around the injection
port/wash station
Sample probe assembly is
bent
Replace sample probe assembly
Clogged wash station • Add water to the injection port/
wash station. Insert cleaning
stylus into drain on side of
wash station.
• Replace wash station assembly
Sheath float switch time
out error during sheath
exchange
Air in the fluid lines • Choose Cytometer > Cleaning
Modes > Prime after Tank Refill.
• Bleed air out of sheath filter.
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