BD FACSCanto - Sample Processing and Modes

Chapter 1: Introduction 23
NOTICE [BD LSR II] Soft standby mode is not available when you are acquiring
samples in Plate mode. Refer to your BD LSR II User’s Guide for information
about soft standby mode.
Sample Processing
During acquisition, the HTS processes samples differently based on the
throughput mode. Table 1-2 compares acquisition in each mode.
Table 1-2 Comparison of acquisition sequence in standard and high-throughput mode
Sequence Standard Mode High-Throughput Mode
1 • raises the probe to the cleaning
position
• flushes the probe using the primary
pump
• turns on the waste pump
• raises the probe to the cleaning
position
• flushes the probe using the primary
pump
• turns on the waste pump
2 • raises the probe
• refills the primary pump
• turns off the waste pump
• draws a separator bubble into the
tip of the probe
• raises the probe
• refills the primary pump
• draws a separator bubble into the
tip of the probe
3 moves the probe and the plate holder
so the probe is positioned above the
required well
moves the probe and the plate holder
so the probe is positioned above the
required well
4 lowers the probe and mixes the
sample
lowers the probe and mixes the
sample
5 aspirates the required sample volume
plus 20 !L of dead volume (required
by the system) along with a separator
bubble
• aspirates a fixed volume of 22 !L
of mixed sample (independent of
the sample volume entered in the
software)
• turns off the waste pump