BD FACSCanto - Page 24

24 BD High Throughput Sampler User’s Guide
6 moves the probe up, over, and down
into the injection port
moves the probe up, over, and down
into the injection port
7 • injects the sample and boosts it to
the cytometer flow cell at high
speed
• a non-selectable, fixed boost
command transports sample to the
flow cell to prime the sample flow
path
• injects the sample and boosts it to
the cytometer flow cell at high
speed
• a non-selectable, fixed boost
command transports sample to the
flow cell to prime the sample flow
path
8 slows down the sample to the
required acquisition speed and
acquires the required sample volume
• delivers the sample to the flow cell
at the required rate and volume
using the secondary (high-
throughput) pump
• simultaneously, raises the probe to
the cleaning position and the
primary pump begins processing
the next well in parallel to
acquisition
9 pushes any remaining sample and the
separator bubble through the flow
cell, and a new cycle begins
once the secondary pump delivers the
sample, pushes any remaining sample
and the separator bubble through the
flow cell. The next well’s sample is
injected and a new cycle begins
10 after the last well in a sequence, raises
the probe to the cleaning position and
performs a final flush and refill
after the last well in a sequence, raises
the probe to the cleaning position and
performs a final flush and refill
11 lowers the probe into the injection
port/wash station
lowers the probe into the injection
port/wash station
Sequence Standard Mode High-Throughput Mode