Chapter 1: Introduction 25
Understanding Volumes
There are different volumes to take into consideration when choosing loader
settings. Figure 1-9 shows different well volumes and Table 1-3 defines each
volume type.
Figure 1-9 Well volumes
Table 1-3 Volume Type
Volume Type Definition
Well volume Volume that well can hold filled to the brim
Total volume Volume pipetted into well – aspirated excess volume
Aspirated excess
volume
Standard mode = 20 !L
Available volume Volume pipetted into well – aspirated excess volume – dead volume
Minimum volume 50 !L for both standard and high-throughput modes for 96-well
plates
Mixing volume Volume one-half the available volume
NOTICE A mixing volume that is larger than the available volume
introduces air bubbles into the sample.
Dead volume Volume in the bottom of the tube that the probe cannot reach.
available volume
plate-dependent dead volume
total volume
well
sample probe
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