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BD FACSCanto - Pausing the BD High Throughput Sampler

BD FACSCanto
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Chapter 3: Running Samples 83
5 Open the safety cover and remove the plate.
NOTICE Access the plate only after sample processing is complete and the
probe is no longer moving.
6 Click the Print button in the Plate toolbar to print a record of the Setup
view and legend.
7 Select the appropriate settings and printer, and then click OK.
Use the printout to
view acquisition run order according to well numbering.
determine well status post-acquisition. (See Plate Layout on page 35.)
8 At the end of each day or shift, clean and service the cytometer as described
in Daily Maintenance on page 104.
Pausing the BD High Throughput Sampler
To pause during a run, click Pause in the Acquisition Dashboard.
The HTS unit finishes processing the current well (or wells in high throughput
mode), and then remains in a suspended state until you choose to continue.
To continue the run, click Resume .
To stop the run, click Stop Well(s) or Stop Plate .
This sequence allows you to stop the run early to minimize additional sample
loss.
Keep a record of acquisition status by printing a copy of the Setup view
immediately after acquisition is complete. Refer to the printout if you
reanalyze or export the data. If you export a data file or an experiment that
contains incomplete data, the software cannot recognize that the file is
incomplete.

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