82 BD High Throughput Sampler User’s Guide
9 Name the compensation setup after the experiment, and click OK.
For information on performing compensation, refer to the BD FACSDiva
Software Reference Manual.
! Tip Compensation controls can be run individually through their normal
worksheets using Basic Controls in the Acquisition Dashboard.
Acquiring Data
Once you calculate compensation, you are ready to record your samples.
1 Click the Worksheets View button to return to the global worksheet.
2 Select well C1 in the plate layout.
3 Click Run Plate in the Acquisition Dashboard.
Wells will be acquired in the order they were created.
As each well is injected into the cytometer SIT, events appear in plots on the
global worksheet. If an error occurs, an error message will appear, and the
well in the plate layout will be colored according to its status. Well status is
indicated by the color outlining each well, as described in Plate Layout on
page 35.
At the end of the run, a dialog appears indicating the run is complete.
4 Click OK to dismiss the completion message.
The HTS is equipped with a safety interlock that prevents the cytometer
from running when the safety cover is removed or opened. Do not remove
or open the safety cover while samples are being processed. To pause or
stop acquisition, click the corresponding buttons in the Acquisition
Dashboard before you remove or open the safety cover.
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