Chapter 5: Troubleshooting 147
Acquisition Troubleshooting
Observation Possible Causes Recommended Solutions
Unexpectedly low event
rate, or no events in
plots
Sheath not pressurized [BD LSR II] Ensure that pressure
relief valve on cytometer sheath tank
is in the closed position.
Sample injection tubing
kinked or damaged
Replace the tubing. See Replacing the
Sample Injection Tubing on page 116.
Sampler coupler tubing
kinked or damaged
Replace the tubing. See Replacing the
Sample Coupler and Tubing on
page 123.
Clogged probe Run the daily cleaning procedure to
clean the probe. See Daily Cleaning
on page 104.
If the probe remains clogged, replace
it. See Replacing the Probe on
page 129.
Clogged sample injection
tubing (SIT)
Check for clogs in the SIT using the
cleaning stylus. See Declogging the
SIT on page 143 for procedure. See
Table A-2 on page 153 for cleaning
stylus ordering information.
If the SIT remains clogged, replace it.
See Replacing the Sample Injection
Tubing on page 116.
Incorrect cytometer
settings
• Ensure cytometer settings are
correct.
• Run setup controls to optimize
cytometer settings for sample type.
Waste tank back pressure • Ensure waste fittings are connected
and not damaged.
• [BD LSR II] Ensure waste lines are
not kinked.
• Ensure antifoam concentrate is
added to waste tank.
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