98 BD High Throughput Sampler User’s Guide
Exchanging the Sheath Fluid on a BD FACSCanto and
BD FACSCanto II
Perform this procedure to exchange one sheath fluid with another. For example,
when switching from plate-based to tube-based acquisition, follow these steps to
exchange BD FACS sheath solution with surfactant with BD FACSFlow.
1 Remove the sample coupler from the SIT.
2 Install a cubitainer containing the sheath fluid you want to use.
3 Empty the waste tank, if necessary.
4 Choose Cytometer > Cleaning Modes > Prime after Tank Refill to display
the Tank Prime window.
5 Select FACSFlow, then click OK.
NOTICE It may be necessary to repeat the prime function multiple times to
remove air from the sheath lines. If air remains in the sheath lines after priming,
purge air from the sheath filter on the wet cart. Refer to your cytometer user’s
guide for more information on troubleshooting air in the fluid lines.
To Next Page
Loading...
