110 BD High Throughput Sampler User’s Guide
- Slide the coupler back up until you reach a hard stop. Make sure the
sample coupler tubing is not kinked or twisted.
- Hold the coupler with one hand while you tighten the tightening nut
with the other hand.
If the coupler is still leaking, replace it. See Replacing the Sample Coupler
and Tubing on page 123.
• Injection port/wash station
Check this area for residual liquid. Liquid appearing around the injection
port/wash station may indicate a clog. If you suspect a clog, refer to HTS
Troubleshooting on page 146.
• Base plate, probe arm assembly, and plate holder
Dampen a clean, lint-free cloth with distilled or DI water and wipe down
only the black surfaces of these components, as needed.
• Sample probe
Make sure the probe is straight. If it is bent or shows signs of wear, replace
the probe. See Replacing the Probe on page 129.
• Pumps
- Wipe up any spills on or around the pumps.
- Inspect the fittings to make sure they are tight. See Inspecting Thumbscrew
Fittings on page 142 and Inspecting Hexagonal Fittings on page 143.
- Inspect the pump syringes for leakage. If your system has been used
extensively and the syringes appear worn, replace them. See Replacing
a Pump Syringe on page 125.
• [BD LSR II and BD FACSCanto] Absorbent pad
Inspect the absorbent pad at the back of the HTS unit to make sure it is not
saturated. Replace the pad, if needed.