Chapter 4: Maintenance 137
1 [BD LSR II] Place the cytometer in Run mode, and ensure that the sample
coupler is installed.
The software automatically primes the HTS pumps during a homing
operation. To prevent pressure from building in the flow cell, the cytometer
must be in Run mode before the homing sequence begins. If you try to run
the Home command when the cytometer is not in Run mode, a software
message will remind you that the fluidic system is not ready.
[BD FACSCanto and BD FACSCanto II] Ensure the sample coupler is
installed and the HTS is turned on.
2 Choose HTS > Home.
3 Verify that the HTS properly performs the following homing sequence:
• The probe moves to the home position, if it is not already there.
• The probe moves up.
• The plate holder moves left, and then slightly forward.
• The probe moves down.
• The plate holder moves right to the home position.
After this sequence, the valves initialize (priming occurs) and the following
message appears.
4 Click OK to dismiss the message.
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