BD FACSort - Page 11

11
BD FACSort System 337452 Rev. A
System Shut Down
Acquisition of flow cytometry data is a multistep process. The following figure outlines
the main steps involved. In this exercise, we will focus on shutting down the cytometer
and the computer.
Always clean the instrument before you power it off at the end of the day. Proper
cleaning will ensure that your instrument will function consistently.
To prevent the sample tube from becoming clogged and to remove adhesive dyes that
could remain in the tubing causing carryover, run a bleach solution through the SIP at
the end of each day followed by a distilled water rinse. Follow this procedure
immediately after running viscous samples or dyes such as propidium iodide (PI),
acridine orange (AO), or thiazole orange (TO).
Cleaning with 10% Bleach
1
Set the fluid control to RUN, install a tube containing 3 mL of a bleach solution
on the SIP with the support arm to the side (vacuum is on), and let it run for
1 minute.
Use BD FACS clean solution or a 1:10 dilution of bleach in DI water as the bleach
solution.
BD FACS clean solution is a bleach-based cleaning agent for daily use in
cytometer maintenance.
2
Move the support arm under the tube (vacuum is off). Allow the bleach solution
to run for 5 minutes on HI.
Rinsing with DI Water
1
Install a tube containing 3 mL of distilled water on the SIP with the support arm
to the side (vacuum is on). Let it run for 1 minute.
2
Move the support arm under the tube (vacuum is off). Allow the water to run for
5 minutes on HI.
3
Set the fluid control to STNDBY.
Start Up
System
Perform
QC
Optimize
Settings
Acquire
Data
Analyze
Data
Shut Down
System