12 BD FACSort System 337452 Rev. A
4 Place a tube containing no more than 1 mL of distilled water on the SIP.
CAUTION Sheath fluid will backflush into the tube and may cause the tube to
overfill if more than 1 mL of distilled water is in the sample tube. This could affect
instrument performance.
5 If you are finished running samples, choose Apple menu > Shutdown to turn off
the computer, and then shut off the power to the cytometer.
The tube of distilled water should remain on the SIP to prevent salt deposits from
forming in the injection tube.
Periodic Maintenance
The following periodic maintenance procedures are necessary to maintain optimal
instrument performance.
• Monthly cleaning
• Sheath filter replacement
• Air filter cleaning
• Bal seal replacement
• Sample O-ring replacement
The following section describes the monthly cleaning procedure. Other procedures can
be found in the BD FACSort User’s Guide.
Monthly Cleaning
Perform overall system fluidics cleaning at least once a month or more frequently if you
are running high volumes of samples or dyes such as propidium iodide (PI), acridine
orange (AO), or thiazole orange (TO).
1 Turn on the cytometer.
2 Remove the sheath tank.
3 Disconnect the upper tubing of the sheath filter from the SALINE FILTER port by
squeezing the metal clip on the quick-disconnect and pulling the connector from
the fitting.
4 Connect the sheath tubing (white) from the tank to the port labeled SALINE
FILTER. See Figure 3-4 on page 13.
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