13
BD FACSort System 337452 Rev. A
This bypasses the sheath filter and allows fluid to travel from the sheath tank
through the tubing directly to the flow cell and to the waste tank.
Figure 3-4 Bypassing the sheath filter
CAUTION Running bleach or detergent through the sheath filter will break down
the filter paper within the filter body. These paper fragments might clog the flow
cell and require replacement of the filter.
5 Install a spare tank with 1 to 2 L of a bleach solution.
Use BD FACS clean solution or a 1:10 dilution of bleach in DI water as the bleach
solution.
6 Press the HI sample flow rate button, then install a tube containing 3 mL of
bleach solution on the SIP.
7 Press the RUN fluid control button and allow the solution to run for 20 to 30
minutes.
8 Remove the tube of bleach solution from the SIP.
OPTIONAL: Repeat steps 5 through 8 using BD FACS rinse solution.
BD FACS rinse solution is a detergent-based cleaning agent.
9 Repeat steps 5 through 8 using DI water.
Replace the tank containing the bleach solution with a spare tank containing 1 to
2 L of DI water. Replace the tube on the SIP with a tube containing 3 mL of DI
water.
10 Remove the tube of DI water from the SIP.
VENT VALVE
PRESS TO RELIEVE
PRESSURE
SALINE
FILTER
J69 J70
WASTE
SHEATH
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