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Beckman Coulter Navios User Manual
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773232AH
October 2015
Instruction F
or Use
Navios Flow Cytometer
CAR
OUSEL CL
EANING:
PREP
ARE A
SO
LU
TION
OF
1
P
AR
T HOUSH
OL
D BLEA
CH
(5%
SOLU
TIONO
F SO
DIUM
HYPOCH
LO
R
IT
E) A
ND
9
P
AR
TS
W
A
TER,
RINSE
CAR
OUSEL
W
ITH S
OL
UTI
ON,
LIG
HTL
Y R
UB IF
NEC
ESSAR
Y
.
IMM
EDI
A
TEL
Y RINSE
WITH
CLEAR
W
A
TER
CAROU
SEL CL
EANING:
PREP
ARE A
SO
LU
TION
OF
1
P
AR
T HOUSH
OLD BLEA
CH (5%
SO
LU
TIONO
F SO
DIUM
HYPOCH
LORIT
E) A
ND 9 P
AR
TS
W
A
TER,
RIN
SE
C
AR
OUSELW
ITH SOLUTI
O
N,
LIG
HTL
Y R
UB IF
NECESSAR
Y
.
IM
MEDI
A
TELY RINSE
WITH
CLEAR
W
A
TER
Beckman Coulter Ireland Inc.
Lismeehan
O’Callaghan’s Mills
Co. Cla
re, Irel
and 353
-65-683
-1100
2
Table of Contents
Revision Status
3
Safety Notice
5
Alerts for Warning, and Caution
5
Safety Symbols
7
Table of Contents
9
Chapter.
34
Introduction
35
Overview
35
Contents
35
How to Find Information
35
Using the System Help
36
Conventions
36
To Choose a Command with the Keyboard
37
Dialog Box
37
Description of Reporting Units
37
Graphics
37
Use and Function
39
System Components
39
Cytometer
40
Supply Cart
40
Workstation
40
Multi-Tube Carousel Loader (MCL)
40
Navios Flow Cytometer Software
41
Options
41
Hardware Options
41
Additional Pmts
41
Printer
41
Workstation/Network Server
41
MCL Carousel Bar-Code Labels
41
Third Laser
42
Reagents and Quality Control Materials
42
Sheath Fluid
42
Cleaning Agent
42
Quality Control Materials
42
Material Safety Data Sheets (MSDS)
43
Installation
45
Delivery Inspection
45
Special Requirements
45
Space and Accessibility
45
Electrical Input
46
Power Consumption
47
Ambient Temperature and Humidity
47
Heat Dissipation
47
Acoustic Noise Level
47
Drainage
47
System Connections
48
Power and Signal Cables
48
Waste and Pneumatic Tubing
49
Power and Signal Cable Connections
49
Waste and Pneumatic Tubing Connections
50
Installing Navios Software
51
Before Installing Navios Software
51
Install Navios Software
51
Operation Principles
55
What this Chapter Explains
55
Sample Flow
55
Sample Loading
55
Hydrodynamic Focusing
55
Laser Beam Shaping
56
Cell Illumination
57
Forward Scatter
57
Side Scatter and Fluorescent Light
57
Laser Beam Shaping
57
Light Collection, Separation and Measurement
58
Forward Scatter Collection
58
Side Scatter and Fluorescent Light Collection
58
Side Scatter
58
Fluorescent Light
59
Side Scatter Collection
59
Two Laser, 6 Color Filter Block Configuration
59
Signal Processing
60
Voltage Pulse Signals
60
Peak Signal
60
Two Laser 8 Color Filter Block Configuration
60
Three Laser 10 Color Filter Block Configuration
60
Voltage Pulse Formation, Peak Signal
61
Integral Signal
62
Integral and Peak Pulses
62
Time-Of-Flight Signal
63
Amplification
63
Time-Of-Flight Pulses
63
Signals Generated
64
Protocols
64
Special Protocols and Panels
64
Special Panels
65
Automated Software Features
65
Parameters
65
TIME Parameter
65
RATIO Parameter
66
Time Vs Fluorescence Plot
66
Plot Display
67
Prism
67
Regions
68
Gating
68
Data Storage
68
Histogram Statistics
69
Linear Region Statistics
69
Log Region Statistics
69
Lin-Log Mean Method
70
Log-Log Mean Method
70
Specifications
73
Sample Requirements
73
Instrument Specifications and Characteristics
73
Dimensions
73
Installation Category
73
Acoustic Noise Level
73
Cytometer
74
Beam-Shaping Optics
74
Blue Laser Beam Spot Size
74
Flow Cell
74
Flow Rate
74
Laser Power Monitoring
74
Lasers
74
Optional Third Laser
74
Sheath Consumption
74
Dynamic Range
75
Laser Beam Separation
75
Optical Filters
75
Red Laser Beam Spot Size
75
Sensors
75
Signal Processing
75
Violet Laser Beam Spot Size
75
Computer
76
Data Storage
76
Digital Accuracy
76
Digital Sampling Rate
76
RAM (Random Access Memory)
76
Workstation
76
Workstation Resolution
76
Input Devices
77
Interfaces
77
Monitor Options
77
Software Specifications
77
Data Output and Compatibility
77
Reporting Units
77
Absolute Count Determination
77
Dual Platform Method
78
Flow-Count (SPT) Method
78
Setup Mode
78
Acquisition
78
Statistics
78
Parameters
79
Regions
79
Listmode Analysis
79
Locked Protocols
79
Default File Extensions
80
Standard (Default) Files & File Extensions
80
Default Autosetup Protocols and Panels
80
Default Generic Panels and Protocols
81
FCS Header - Keyword Reference
82
Fcs 2.0
82
FCS 2.0 Keyword Information
83
Acquisition Protocol Section
86
FCS 3.0 Section
86
FCS 3.0 Keyword Information
87
Database Structure
89
Firewall Setup
89
Database Login
92
Report Generator Database Schema
92
Cytoimage Table
92
Cytoinfo Table
92
Cytorun Table
93
Table
93
Cytosamples Table
95
Cytospecimen Table
95
Cytotemplate Table
96
Installationid Table
97
Cytotemplate_Line Table
97
Instrument_Directories Table
98
Instrument_Installation
98
Instrument_Signals
98
Instrument_Statistic
98
Lis_Transfer
98
Qc_Base Table
99
Qc_Comment Table
101
Qc_Instance Table
102
Qc_Maintenance Table
102
Qc_Mainttype Table
103
Qc_Products Table
103
Qc_Servicelog Table
103
Qc_Template Table
103
Settingstable
104
Performance Characteristics and Specifications
105
Carryover
105
Acquisition Rate
105
Data Acquisition Throughput
105
Precision for Surface Markers
105
Scatter Resolution
106
Fluorescence
106
Forward Scatter
106
Sensitivity
106
Laser Characteristics
106
Day-To-Day
106
Within Day
106
System Overview
107
Navios Intended Use and System Description
107
Intended Use
107
Cytometer Controls and Indicators
108
Navios Flow Cytometer
108
Cytometer Indicator Panel
109
Signal Amplitude Indicators
110
Cytometer Ready and Sheath Flow Indicators
110
Level Sense Indicators
111
MCL (Multi-Tube Carousel Loader)
111
Workstation
111
Supply Cart
112
Printer (Optional)
112
Microsoft Windows Desktop
112
Windows Administrator Password
112
Taskbar
112
Learning the Basic Operating Techniques
113
MCL Carousels
113
Bar-Code Labels
113
Putting a Bar-Code Label on a Sample Tube
114
Putting Sample Tubes in a Carousel
115
Putting a Carousel in the MCL
115
Removing a Carousel from the MCL
117
Navios Software Shortcuts
118
Keyboard Shortcuts
118
Drag and Drop
119
Drag and Drop Table
119
Toolbar Buttons
120
Creating Protocols
121
Creating Regions
122
Create Polygonal Regions
122
Create Rectangular Regions
123
Create Quadrant Regions
124
Repositioning a Quadrant Region
125
Create Linear Regions
126
Create Multiple Linear Regions
127
Creating Gates
127
Create Autogate
127
Automatic Gate Creation
130
Convert a Polygonal Region to an Autogate Region
130
Creating Flowpages
131
Flowpage Example
131
Delete Flowpages
132
Creating Panels
133
Use Plots and Gates from Previous Test
135
Use Plots and Gates from Protocol
135
Use Instrument Settings
135
Use Regions from Protocol
135
Carry Regions from Previous Protocol
135
Use Regions from this Protocol
135
Use Instrument Settings from Previous Test
135
Use Instrument Settings from External File
136
Parameter Names
136
Creating Worklists
136
Before Creating Worklists
136
Worklist Panel
137
Worklist Test
137
Verifying a Worklist
137
Using the System Help
138
Access System Help
138
How to Use Help
138
Daily Routine
141
Before You Begin
141
Daily Startup
141
Check Waste and Reagent Levels
142
Power the Computer and Cytometer on
143
Turning on Power
143
Logging Onto Navios Software
144
Logging Onto Windows Software
144
Additional Start up Checks
146
Daily Shutdown
146
When to Shut down the Cytometer
146
Before Performing Shut down
146
Power the Computer and Cytometer off
147
After Instrument Shut down
148
Extended Shutdown
148
Cytometer Auto Startup
152
Quality Control
155
Introduction
155
Instrument Setup
155
Parameters
155
Angle of Forward Light Scatter
155
Discrimination Parameter
156
Discriminator Value
156
Flow Rate
156
Voltages and Gains
156
Plot Selection
156
Regions and Gating
156
QC Processes
156
Daily QC
157
Preparing Autosetup Samples
157
Autosetup Scheduler
158
Wizard
161
Before Running the Autosetup
161
Wizard
164
Application Definition Wizard
167
Before Running the Application Definition Wizard
167
Target Value Entry Description
174
Laser Selection
175
Setting up QC Products
175
Products
176
Review QC Results
177
Running Samples
179
Sample Requirements
179
Before Running Samples
179
Running Samples - MCL Automatic Mode
180
Verifying a Worklist
180
Running Samples - MCL Manual Mode
184
Running Samples - Single Tube Mode
189
Data Review
193
Review Data Displays
193
How to
195
How to
195
Software Procedures
195
These Procedures Are in the Quality Control Chapter
195
These Procedures Are in the Using Navios Software Chapter
195
Hardware Procedures
196
Using Navios Software
197
Welcome to Navios Software
197
Multi-User Sign on
197
Overview
197
Configure System Administrator
197
Cytometer Startup Page
197
Admin Button
199
Logged in
199
Next Button
199
Password
199
User ID
199
Connect
200
Work Offline
200
Sign-On Administration
200
User Administration
200
System Administrator Screen
201
Add User
201
Delete User
201
Edit Button
202
Modify User
202
Users
202
Workgroup Selection
202
Workgroups
202
Edit Workgroups
202
Delete
203
Export Log
203
Add
203
User Account Control
206
System Configuration
206
Report Generator Database Utilities
206
Backup
206
Name
206
Destination
207
Comment
207
Archive Data Screen
207
Archive Location
207
Archive Criteria on or before [Date]
207
Archive Criteria between [Date] and [Date]
207
Archive QC Data
208
Archive Maintenance Details
208
Archive Service Details
208
Archive Panel Reports
208
Patient Name
208
Patient ID
208
Sample ID1
208
Panel Name
208
Restore Archive
208
User Profile - User ID
209
User ID
209
First Name
209
Last Name
210
Title
210
Privileges
210
System Administrator
210
Lock / Unlock Protocols
210
User Configuration
210
Access Other Files
210
Remember Last Accessed LMD Directory
210
Automatically Overwrite Acquired Data Files
210
Overwrite Other Data Files
211
Add Absolute Calibration Batches
211
Can Modify Custom Dyes
211
User Status
211
Change Password
211
User Profile - Paths
211
Browsing Buttons
212
Home
212
Path Details
212
Backup
212
User to Back up
213
Directories List
213
Overwrite Existing Backup Files
213
Move Local HST Files to the Recycle bin
213
Move Local LMD Files to the Recycle bin
213
File Options Toolbar
213
Plot Options Toolbar
214
Regions Options Toolbar
215
Gate, Color, Stats and Help Toolbar
215
Flowpage Toolbar
216
Acquisition Manager Toolbar
217
Automator Toolbar
217
Cytometer Toolbar
218
Listmode Playback Toolbar
219
Report Generator Toolbar
220
File Menu
221
New Protocol
221
Creating a New Acquisition Protocol
222
Creating a Protocol for Analysis
222
New Panel
224
New Worklist
224
Open Listmode File
224
Using Drag and Drop
225
Using the File Menu
225
Save Listmode File as
226
File Name
227
Save Histogram File as
227
Save All FCS Format Files
228
Open Protocol
229
Save Protocol
229
Save Protocol as
229
Lock Protocol
230
Unlock Protocol
230
FCS Keywords Used in Navios Software
231
File Name
231
Save as Type Combo Box
231
Save Button
231
Save in
231
Save Listmode Compensation as
231
View FCS Header
231
Edit FCS Header Attributes
232
Import
233
Settings from CMP File
233
Print
234
Print Flowpages
234
Print Plots
234
Print Statistics
234
Print to PDF
235
Workspace Preferences
235
Workspace Preferences - LMD File Name
236
Characters
237
Sample ID
237
User ID
238
Tube ID
238
Automatically Add Unique
238
Y2K Date
238
Time
238
Tag Number
238
File Name Extension
239
PDF File Name Extension
239
Append .PDF File Name
239
Replace
239
Workspace Preferences - Acquisition Options
239
Output Options
240
Acquisition Manager
240
Cytometer Control Translucency
241
Workspace Preferences - User Info
241
Institute
242
Institute Street
242
Institute Town
242
Sample Source
242
Project
242
Cell Type
243
Experiment
243
Cytometer Serial Number
243
Currently Logged on User
243
Workspace Preferences - Plot Display
243
Show % in Region on Plots
244
Black Plot Backgrounds
244
Opaque Region Labels
244
Print Plot Frames
244
Print Dots Black
244
Log Scale Density Plots
244
Smooth Histograms
244
Default % of Events on Dot Plot
244
Baseline Offset
245
Default Plot Size
245
Workspace Preferences - Gating
246
Automatic Gate Creation
246
Automatic Gate Maintenance
246
Automatic Color Precedence
246
Dot Plot Coloring Mode
247
Autogating Sensitivity
247
Elliptical Sensitivity
247
Contour Sensitivity
247
Abort on Error
247
Autogating Failure
247
Contour Travel
247
Ignore Errors
247
Pause on Error
247
Do Not Show Warnings
248
Live Gate Warnings Options
248
Show Warnings Radio Button
248
Workspace Preferences - Publish
248
Auto-Close Excel Workbook
249
Export Bitmap Size
249
Export Data Format
249
File References in Data
249
Publish Data to MS Excel
249
Publish Data to Text File
249
Switch to MS Excel on Publish
249
Exit
250
Log out
250
Edit Menu
250
Undo
250
Redo
250
Cut
250
Copy
251
Paste
251
Paste Special
252
View Menu
252
Acquisition Manager
253
Acquisition Manager Docking Options
253
Allow Docking
253
Hide
253
Acquisition Manager - Modifying Panels and Worklists
254
Float in Main Window
254
Panels
254
Worklist Columns Available
255
Worklists
255
Acquisition Manager - Panel
256
Acquisition Manager - Plots
256
Acquisition Manager - Protocol
256
Acquisition Manager - Region Sources
257
Acquisition Manager - Cytosettings
258
Acquisition Manager - Parameter Names
258
Acquisition Manager - Carousel No. and Location
259
Acquisition Manager - Tube ID
259
Acquisition Manager - CAL Factor
260
Acquisition Manager - LMD File Name
260
Acquisition Manager - Sample ID
260
Acquisition Manager - Stop Condition and Stop Value
261
P1-P32
261
Resource Explorer
261
Toolbars - Customize Toolbars
262
Cool Look
262
Show Tooltips
262
Toolbar Name
262
Toolbars
262
Large Buttons
263
New
263
Reset
263
Toolbars - Customize Commands Tab
263
Buttons
263
Categories
263
Description
263
Zoom
264
Customize Worklist Columns
264
Reordering Columns
264
Carousel Overflow
265
Deleting a Worklist Row
265
Insert Menu
265
Blank Flowpage
266
Protocol Status
266
Flowpage from File
267
Flowpage Plot
268
Insert Plot
268
Inserting Drag & Drop Plot
268
Flowpage Statistics Table
269
Flowpage LMD Compensation Grid
269
Flowpage Textbox
269
Other Flowpage Object
270
Inserting a Rectangle
270
Inserting a Line
271
Inserting a Picture
271
Inserting a Text File
272
Tools Menu
274
Publish Results Now
274
Open Excel Workbook
275
Open PDF
275
Open Text File
275
Automator
275
Welcome to Automator
275
Automator Setup
276
Add Blank
278
Add Files
278
Load Queue
278
Remove Files
278
Reordering Files
278
Automator Status Bar
279
Output Options
279
Print
279
Restore Analysis Files on Startup
279
Save Queue
279
Show Full Pathname
279
Listmode Playback
280
Single File Mode
280
List Comp Setup
281
Options Tab
282
Autosetup Application Definition
283
Autosetup Scheduler
283
Custom Dye Entries
284
Plots Menu
284
Plots - Introduction
284
Duplicate Plot
285
Color Dot Plot
285
Copy Plot Data
286
Copy Plot Image
286
Dot Plot Data Source
286
File
286
Format Plot
286
Gate
286
And y Parameter
287
Apply % on Plot to All Plots
287
Apply Gate to All Plots
287
Dot Plot Events
287
Show % on Plot
287
Apply to All Plots
288
Dot Plot Labeling
288
Dot Plot Resolution
288
No. of Events to Show
288
And y Axis Labels
289
Show Axis Labels
289
Show Axis Tick Marks
289
Show Plot Title on Output
289
Histogram Plot
290
Histogram Plot Configuration
291
Histogram Plot Data Source
291
Parameter
291
Smooth
291
Use Gate Color
291
Freeze Frame
292
Histogram Freeze Frame
292
Create a Histogram Freeze Frame
293
Delete a Histogram Freeze Frame Overlay
293
Automatic Maximum Value
294
Clip Channels
294
Histogram Scaling
294
Density Plot
295
Histogram Labeling
295
Set Maximum
295
Density Plot Data Source
296
Automatic Maximum Threshold
297
Density Level Colors
297
Density Level Configuration
297
Logarithmic Threshold Calculation
298
Maximum Density Level
298
Smooth Density Plot
298
Use Gate Color
298
Use Single Color Range
298
Density Plot Labeling
299
Density Plot Resolution
299
Prism Plot
299
Data Source (Prism)
300
What Is Prism
300
How Is Prism Created
301
Prism Dividers
301
Setting a Single-Parameter Prism Divider
301
Dual Parameter Prism
302
Editing Prism Dividers
303
Navios Software - Prism Compatibility
303
Removing Prism Dividers
303
Legend
304
Data Source (Legend)
305
Gated
305
Legend - Options Tab
305
Number
305
Show "Phenotype Logic" Columns
305
Total
305
Absolute Count
306
FCS Information
306
Info Plot - FCS Tab
307
Info Plot Data Source
307
Acquisition Stop and Save
307
Setting a Stop Counter
308
Use Stop Condition
308
Maximum Events
309
Save Histogram Data in FCS Format
309
Format Plot
310
Analysis Menu
310
Create / Modify Gates
311
Gate and Logical Expression
311
New
311
Delete
312
Operands
312
Gate Selection
312
Gate Color
312
Boolean Gating
313
Gate Logic
314
Color Blend Mode - Introduction
315
Modify Color Blend
316
Enable
317
Gate Names
317
Blend Color
318
Color
318
Create Gate
318
Logic
318
Modify Color Precedence
318
Advanced Precedence
319
Polygonal Region
320
Region Properties
320
Regions Introduction
320
Automatic Region Name Positioning
321
Contour
321
Elliptical
321
None
321
Polygonal Region Properties
321
Prime
321
QC Product
321
Region Name
321
Region Statistics Exported for Quality Control
321
Sensitivity
321
Interactive Polygonal Region Editing
323
Linked Region
323
Minimum Count
323
Polygonal Region Format Tab
323
Rectangular Region
323
And y Coordinate
324
Interactive Rectangular Region Editing
324
Quadrant Region
324
Rectangular Region Properties
324
Contour Autogate
325
Elliptical Autogate
325
Interactive Quadrant Region Editing
325
Linear Region
325
Quadrant Region Properties
325
Interactive Linear Region Editing
326
Linear Region Properties
326
Lower Limit and Upper Limit
326
Positives Analysis
326
Status Bar (Linear)
326
Target Region for Automatic Setup
326
Coordinate
327
Integral
327
Interactive Multi-Linear Region Editing
327
Multiple Linear Regions
327
View / Modify Regions
327
Region Name
328
Region Points
328
Resizing a Region
329
Moving a Region
329
Editing and Moving a Region Name
329
Deleting Regions
330
From Within a Plot
330
Using the Delete Button in the View / Modify Regions Dialog Box
331
Re-Calculate All Autogates
331
Create Prism Divider
331
Dual Prism Divider
331
Setting a New Dual Prism Divider
332
Editing a Dual Prism Divider
332
Single Prism Divider
333
Setting a New Single Prism Divider
333
Editing a Single Prism Divider
333
View/Modify Prism
334
Select Results
335
Statistic Type
336
Mean Calculation Method
336
FCS Information
336
Number
336
Total
337
Gated
337
Median
337
Hp-CV
337
Mode
338
Cells / Μl
338
Min and Max
338
Select Numeric Precision
338
Enter Numeric Value
338
Advanced Statistics Configuration
338
Differences between Log-Log and Log-Lin Statistical Calculations
338
Log-Log
338
Log-Lin
339
Automatic Statistic Percentiles
339
Absolute Count Calibration
339
Setting a CAL Factor for Absolute Counts
339
Override Runtime CAL Factor
340
Replace
340
Delete
340
Setting a CAL Region
340
Calculate Results
340
Refresh
340
Add
340
Enable Trueview Checkbox
341
Trueview Setup
341
LMD Quickcomp
342
Negative Decades Slider
342
Set 1 - 5 Radio Buttons
342
Show Analysis Algorithm Results
343
Show LMD Quickcomp Matrix
343
Flowpage Menu
343
Flowpage - Introduction
344
Editing Flowpages
344
Move a Selected Item Within Flowpage
344
Select a Single Item Within Flowpage
344
Additional Flowpage Formatting Options
345
Deleting Objects in Flowpage
345
Resizing Objects in Flowpage
345
Select a Group of Items Within Flowpage
345
Change Plot
348
Edit Text
348
Grow to Largest
349
Shrink to Smallest
349
Align Bottom
350
Align Left
350
Align Right
350
Align Top
350
Save Flowpage as
351
Space Evenly Across
351
Space Evenly down
351
Do Not Show Plot Events
352
Cytometer Menu
353
Cytometer Control
353
Cytometer Control Acquisition Setup Tab
354
Discriminators
354
Label
354
Details
355
Dots
355
Gain
355
Setup Mode
355
Volts
355
Baseline Offset
356
Quickcomp Mode
356
Quickset Mode
356
Acquisition Time
357
Elapsed Time
357
Limits
357
Parameter Button
357
Set Acquisition/Elapsed Time
357
Cancel
358
Live Gate
358
Max Acquirable Events
358
Maximum Events
358
Cytometer Control Parameter Setup
359
Clear All Button
360
Collection Angle
360
Compensation Matrix
360
Cytometer Control
360
Cytometer Control Acquisition Setup Tab
360
Cytometer Control Compensation Tab
360
ND Filter
360
See also
360
Status Page
360
Cytometer Log
361
Get Cytosettings from Protocol
361
Abort
362
Get Cytosettings from LMD File
362
Pause
362
Stop
362
Abort the MCL / Worklist
363
Abort the Panel
363
Abort the Tube
363
Repeat the Panel
363
Repeat the Tube
363
Restart
363
Cleanse
364
Idle Mode
364
Laser Control
364
Pause and Rotate
364
Acquisition Time/Elapsed Time
365
Cytometer Status
365
Event Rate
365
Status Bar
365
Cytometer Error/Warning Messages
366
Flow Rate
366
Number of Events
366
Windows Status Bar
366
And y Coordinates
367
Window Menu
367
Cascade
367
Tile
367
Tile Special
367
Arrange Icons
369
Force Square
369
Close All
369
Help Menu
370
Navios Help
370
About
370
System Options
370
Hardware Group
371
Hardware Group - Firmware
371
Hardware Group - Lasers
371
Hardware Group - Pmts
371
Software
371
Description Field
372
Installed Software Modules Group
372
List Control
372
Online Support
372
Quality Control Report
372
QC Levey Jennings Screen
373
Application
373
Data Table
373
Instrument
373
Remove Points
373
Reference Points
374
Delete Plot
375
New Plot
375
Clear Template
376
Close
376
File Menu
376
Page Setup
376
Print
376
Print Preview
376
Save Template
376
Save Template as
376
Select Template
376
Template Menu
376
Modifying Template Files
378
Select Parameter
378
Select Mean
379
QC Data Table
380
Select Limits
380
Create Spreadsheet File
381
File Menu
381
Page Setup
381
Print
381
Print Preview
381
Edit IQAP
382
IQAP Menu
382
Panel Report Template
382
Overview
382
Creating and Importing Panel Report Templates
383
Create a CPF File
383
Create the Panel Report Template
385
Importing a Panel Report Template
385
Cytometer Serial Number
386
Panel Name
386
Panel Path
386
Verifying a Panel Report Template
387
Global Tab Settings
387
Local Tab Settings
388
Data Plots Tab Settings
391
Editing Panel Report Templates
391
Before Running an Export Panel for the First Time
391
Procedure for Editing/Saving Panel Report Templates
391
Panel Report
395
Printing Panel Reports
399
Auto-Printing
399
Manually Printing Panel Reports
399
Tips for Selecting Reports
401
Editing Report Comments
402
Patient Database Editor
404
Entering/Editing Database Information
405
Database Management
405
Archive Data
407
Restore Archive
409
Report Generator Error Log
410
Error Log Screen
411
Error Messages List
411
Print
411
Save
411
Cleaning Procedures
413
What this Chapter Explains
413
Cleaning Schedule
413
Clean the Air Filters
413
Location of Air Filters
414
Prepare to Clean the Air Filters
414
Rinse and Return the Air Filters
415
Filters
416
Put the Cytometer in the Idle Mode
417
Remove the Reagent Containers
418
Mode
418
Clean the Internal Sheath Fluid Container
420
Container
422
Clean the Cleaning Agent Container
423
Replace the Reagent Containers
426
Clean the Sampling System
428
When to Clean the Sampling System
429
Clean the MCL Sample Head and the Sample Probe
429
System
430
System
431
Routine Cleaning Procedure
436
Testing for Residual Stain
439
Clean the Vacuum Trap
440
Prepare to Clean the Vacuum Trap
440
Find and Pull out the Vacuum Trap
441
Rinse and Return the Vacuum Trap to Its Bracket
442
Trap
442
Power the Cytometer Only On/Off
444
Power the Cytometer Only on
444
Power the Cytometer off
444
Replace/Adjust Procedures
447
What this Chapter Explains
447
List of Replacement and Adjustment Procedures
447
Replacement/Adjustment Schedule
447
Replace Reagents
448
About the Reagent Containers
448
Reagent Container Capacity
448
Cleaning Agent Container
448
Replace the 10 L External Sheath Fluid Container
448
Fill the Internal Sheath Fluid Container
453
Fill the Cleaning Agent Container
457
Empty the 20L Waste Container
460
Replace the Sheath Fluid Filter
463
Replace the Sample Probe and Sample Pickup Tubing
466
Replace the Mcl Sample Head
475
Adjust the System Pressure
482
Remove Filter Holder
485
Replace Dichroic Filter
487
Replace Bandpass Filter
489
Identify Coated Side of Dichroic Filter
490
Replace Filter Holder
491
Troubleshooting
493
Precautions/Hazards
493
Laser/Radiation Precautions
493
Laser Warning Labels
493
Laser Labels on the Sensing Compartment Cover
494
Laser Labels in the Sensing Compartment, Cover Removed
495
Labels on the Lasers in the Sensing Compartment, Cover Removed
496
Laser Labels on the Filter Array, Cover Removed
497
Laser Label on the Cytometer Back Panel
498
Laser Labels on the MCL Bar-Code Reader
499
Warning Labels on UPS
500
International Warning Symbol Locations
500
Disposal of Electrical Instrumentation
501
Rohs Caution Label
501
Rohs Environmental Label
501
Disposal Precaution
502
EMC Information
502
Maintenance Schedules
502
Cleaning Schedule
502
Replacement Schedule
502
Cytometer Messages
502
Display Locations
503
Cytometer Status Messages Screen
503
Navios Software Screen
503
Error Message on Navios Software Screen, Example
503
Cytometer Status Messages Window, Example
503
Instrument Setup
504
Navios Message Display Location Summary
504
Cytometer.log File
505
How to Access the Cytometer.log File
505
From Navios Software
505
From Windows Desktop
506
Cytometer.log Entry Description
506
How to Search the Cytometer.log File
506
Other Functions Available
506
Change Font
507
Find Help Topics
507
Cytometerarchive.log File
507
Cytometer Messages Table
507
Table 13.2
507
Software Messages
517
Navios Software Messages
517
Table 13.3
517
Operating System Error Messages
519
Level Sense Indicators
519
Sheath Low
519
Sheath Low Indicators
519
Waste Full
520
Cytometer Control Window Cannot be Viewed
520
Waste Full Indicators
520
Bar-Code Specifications
523
Bar-Code Sample Identification
523
Bar-Code Label
523
Correct Placement of the Bar-Code Label
524
Bar-Code Label Specifications
524
Bar-Code Label Placement
524
Label Size and Thickness
525
Symbol Dimensions
525
Label and Print Quality
525
Bar-Code Label Specifications
525
Bar-Code Error Rate
526
Bar-Code Symbologies
526
Bar-Code Labels
527
Bar-Code Symbologies
527
Bar-Code Label Optical Characteristics at 670 Nm ±10
528
Mcl Bar-Code Reader
528
Bar-Code Decoder
528
Checksum Algorithm
529
Maintenance and Service Logs
531
Maintenance Log
532
Menu Options
532
Admin
533
User Entry
533
Administrator Entry
534
Service Log
536
Menu Options
536
Enter Condition Noted
537
File Menu
537
Navios Maintenance Log
537
Using the Service Log
537
Enter Action Taken
538
Start Date: and End Date
538
References
539
Glossary
541
Index
547
Before You Begin
556
Mode
558
System Components
577
4
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Beckman Coulter Navios Specifications
General
Brand
Beckman Coulter
Model
Navios
Category
Measuring Instruments
Language
English
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