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LS 13 320
Beckman Coulter LS 13 320 User Manual
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PN B05577AC
July 2017
User’s Manual
LS 13 320 Laser Diffraction P
article Size Analyzer
Beckman Coulter, Inc.
250 S. Kraemer Bl
vd.
Brea, CA 92821 U.S.A.
2
Table of Contents
Default Chapter
3
Revision History
3
Safety Notice
5
Table of Contents
13
Introduction
23
CHAPTER 1 Theory
31
Scattering Patterns for Spheres
32
Polarization Intensity Differential Scattering (PIDS)
33
3.1.4 Particle Non-Sphericity & Light Scattering
34
Laser Diffraction Measurement for Non-Spherical Particles
34
Scattering from Different Polarizations
34
PIDS Sizing
35
Components
35
Functions
35
Description
36
Theoretical Background
31
Light Scattering
31
Mie Theory
32
3.1.2 Fraunhofer Theory
32
CHAPTER 2 Installation
37
Installation Requirements
37
Environment
37
Power Requirements
37
Temperature and Humidity Requirements
38
Sample Modules Specific Requirements
38
Suspension Fluid for Aqueous and Universal Liquid Modules
38
Electrical Specification Labels
38
Drain for Aqueous Liquid Module
39
Drain for Universal Liquid Module
39
Vacuum for Tornado Dry Powder System
39
Hardware Connections
39
Location of Interunit Connections
39
Configuring Software Parameters
40
Connections Dialog Box
40
Software Installation
40
Installing and Starting the Software
40
Getting Started
41
Starting the Instrument and Its Control Program
41
Instrument Verification
41
Post-Installation Verification
41
Daily Verification
41
Security Level Options
42
Setting up for Compliance
43
User Set-Up Flowchart
43
Selecting a Module
46
Message When an Optical Module Is Not Found
47
Ready Screen
47
Selecting an Optical Module
47
Making Measurements
48
Measuring Offsets
48
Alignment
48
Measure Background
48
Measure Loading
48
Running a Control
49
Run Menu
49
Run Menu
50
Auto Align Screen
51
Measuring Offsets Screen
51
Measuring Obscuration (Loading) Screen
52
Measuring Offsets Screen
52
Run Settings Dialog
53
Running a Sample
54
Reference Background
55
Sample Info Dialog
53
Optical Model Dialog Screen
55
Run Cycle Options Dialog
57
Reference Background File Selection
58
Chapter 3
59
Software
59
File Menu Options
60
LS 13 320 Software
61
Window Menu Options
69
Run Menu Options
70
Preference Options
75
Printed Report
75
Preference Files
88
Security Options
90
Security Setup
92
5.5.2 Types of Users
93
Administrator
93
Add a User Account
96
Edit a User Account
97
View User Account Info
97
Supervisor
98
Customize Main Menu
98
Customize Run Menus
99
Customize Sample Info Names
99
Recent Files and Directories
100
No Security
100
Menu
100
Low Security
101
User Privileges Dialog: no Security
101
Account
101
Medium Security
103
User Privileges Dialog: Low Security
103
High Security
104
User Privileges Dialog: Medium Security
104
User Privileges Dialog: High Security
105
Logging out
106
CHAPTER 4: Regulatory Compliance - 21 CFR Part
107
Electronic Record Control - 21 CFR Part 11
107
File Historyand Tracking
107
Audit Trail
109
Electronic Signatures
110
Generating Electronic Signatures
111
Technique
111
Applying Electronic Signatures
113
Additional Security Features
114
Data Mirroring
114
File Attributes
114
Starting Security-Enabled Software
115
CHAPTER 5: Data File Menus
117
Save
119
Export
119
Export Data Dialog Box
120
Preferences Data Dialog Box Exporting Data
121
Get Info
122
Get File History
122
Edit Menu
123
No Security
123
View Menu
125
Graphs
125
Display Values on One Channel
126
Display Multiple Channel Values
126
Expand Part of a Graph
126
Listing
127
Analyze Menu
128
Statistics
128
Statistics Information
128
Compare to Standard
129
Compute Sizes
129
Select Optical Model Dialog Box
129
Data File Menus
117
Runfile Menu
117
Open for Overlay
117
CHAPTER 6 Sample Modules
131
Aqueous Liquid Module
131
Aqueous Liquid Module (ALM) with Sample Cell
131
ALM Description
132
System Description
132
Indicators
132
Liquid Level Sensors
132
Software
133
Connecting the ALM
134
Recirculation Lines
134
Inlet and Drain Hoses
134
Loading the Sample Cell into the Optical Bench
135
Software
136
8.1.3.1 Control Menu
137
Standard Operating Methods (SOM)
138
8.1.3.3 Loading an SOM
142
Standard Operating Procedures (SOP)
143
Suspension Fluids
144
Cleaning the ALM
145
Replacing the Diffraction Cell Window
147
ALM Specifications
151
ALM Troubleshooting
151
Auto-Prep Station
151
APS Description
152
Connecting the APS
152
Software
154
Run Menu
154
File Menu
155
Edit Menu
156
8.2.3.1 APS Run Menu
156
Auto-Prep Station Settings
157
8.2.3.2 APS Select Menu
159
8.2.4 APS Troubleshooting
160
Micro Liquid Module
160
MLM Description
161
Loading the MLM
162
Software
162
Control Menu
162
Stirrer Speed
163
Stirrer On/Off
163
Standard Operating Methods (SOM)
163
Creating Soms
163
Indicators
163
Loading an SOM
168
Software
168
Standard Operating Procedures (SOP)
170
Creating an SOP
170
Loading an SOP
171
Suspension Fluids
172
MLM System Preparation
174
Placing the Cell on the Sample Stand
174
Filling Micro Liquid Module with Diluent
175
Adding Sample
176
Setting the Stirring Motor
176
Cleaning the MLM
177
Clean the Cell Components
177
MLM Troubleshooting
179
Tornado Dry Powder System
179
Tornado Description
180
Indicators
180
Docking the Tornado DPS
181
Software
181
8.4.3.1 Control Menu
182
Standard Operating Methods (SOM)
183
Creating Soms
183
8.4.3.3 Loading an SOM
186
Standard Operating Procedures (SOP)
187
Creating Sops
187
Loading an SOP
188
Cleaning the Tornado DPS
188
Using the Cleaning Sample
188
Cleaning the Cell Windows
189
Cleaning the Sample Tubing
191
Cleaning the Sheath Flow Filter
193
Tornado DPS Troubleshooting
194
Tornado DPS Specifications
196
Universal Liquid Module
196
ULM Description
197
Loading the ULM
197
ULM Software
198
8.5.3.1 Control Menu
198
Sop)
199
Standard Operating Methods (SOM)
200
8.5.3.3 Loading an SOM
204
Standard Operating Procedures (SOP)
205
Suspension Fluids
206
Cleaning the ULM
208
Cleaning Fluids
208
Window
208
Cleaning the Cell
209
Replacing the Diffraction Cell Window
211
Sample Cell Windows
213
ULM Troubleshooting
215
ULM Specifications
215
Connections
215
Module
216
APPENDIX A: LS 13 320 Specifications
217
Specifications
217
APPENDIX B: Sample Handling
219
Material Handling
219
Liquid Sampling
219
Solid Sampling
219
Technique
219
Spinning Riffler
220
Chute Splitter
220
Scoop Sampling
222
Cone and Quartering
222
Liquid Sample Dispersion
222
Physical Methods of Liquid Dispersion
223
Chemical Methods of Liquid Dispersion
224
Stabilization by Surface Charge
224
Steric Stabilization
225
Physical and Chemical Methods of Liquid Dispersion
225
Special Cases
225
A Practical Recipe for Liquid Sample Dispersion
225
Solid Sample Dispersion
226
Diluent Selection
226
Consider Solvation Effects
226
Consider Other Effects
226
Consider the Effects the Diluent Has on the Sample's Suspension Fluid
226
Consider Characteristics of the Diluent
227
Viscosity
227
Active Life
227
Reactivity
227
Purity
227
Refractive Index
227
Color
227
APPENDIX C: Optical Models
229
Introduction
229
Refractive Index
229
Extended Optical Model
230
Creating an Optical Model
230
Creating an Optical Model from the Runfile Menu
231
Dialog
232
Statistics
233
User-Defined Mean
233
Dialog
233
Median and Mode
234
Median
234
Mode
234
Characterizing the Distribution
234
Variance
234
Kurtosis, C
234
Standard Deviation
235
Coefficient of Variation
235
Skewness
235
Kurtosis
236
APPENDIX D: Troubleshooting
239
Background
239
Contaminants
240
Troubleshooting High Backgrounds
241
Reference Background Set-Up
241
Background, D
241
Part 1 - Setting up a Reference Background
242
Part 2 - Using the Reference Background
246
APPENDIX E: Maintenance
247
Cleaning Procedures
247
Cleaning Fluids
247
Cleaning the Lens
247
Windows
247
Fourier Lenses
248
Projection Lens
249
Beckman Coulter, Inc
251
Customer End User License Agreement
251
Test
251
Aps
252
Related Documents
254
Menu
254
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Beckman Coulter LS 13 320 Specifications
General
Brand
Beckman Coulter
Model
LS 13 320
Category
Measuring Instruments
Language
English
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