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PA 800 plus
Beckman Coulter PA 800 plus User Manual
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A51964AC
February 2011
User’s Guide
P
A 800
plus
Pharmaceutical Analysis
System
System Maintenance
Beckman Coulter, Inc.
250 S. Kraemer Bl
vd.
Brea, CA 92821
2
Table of Contents
Default Chapter
3
Revision History
3
Safety Notices
5
Symbols and Labels
5
Table of Contents
11
Introduction
13
Install UV or PDA Detectors
14
Calibrate the PDA Detector
17
Install UV Detector Wavelength Filters
18
Integrated 488 Nm Solid-State Laser
24
Installation Requirements
24
Install the Solid-State Laser Module
24
Install the LIF Detector Filters
25
Install the Filter Housing into the Detector
26
Install the LIF Detector into the PA 800 Plus
27
Connect the Probe to the Clamp Bar
27
LIF Detector Calibration
29
Troubleshooting Procedures
32
Capillary Cartridge
33
Rebuild a Capillary Cartridge
33
Install a Capillary Cartridge into the PA 800 Plus
51
Universal Vials and Caps
54
Fill the Universal Vials
54
Fill the Micro Vials
55
Maintenance Procedures
57
Opening Levers, Electrodes, and the Interface Block
58
Clean Electrodes, Opening Levers, and the Interface Block
61
Coolant
62
Fiber Optics (UV and PDA Detectors)
64
Detectors)
65
Lamp
66
Instrument Care
73
LIF Care and Maintenance
73
Inspect the LIF
73
How to Clean the LIF
73
How to Store the LIF
73
Replace the Quad-Rings
74
Replace the Fuses
75
CHAPTER 3: Interfacing PA 800 Plus with Mass Spectrometry
77
CE-MS/MS Mode Cartridge Procedures
77
How to Install the Capillary in the Standard Cartridge
78
How to Install the Capillary in the EDA-Only Cartridge
79
Ground Connection
81
APPENDIX A: LIF Test MIX Calibration and Performance Tests
83
Relative Fluorescent Units (RFU
83
Calibration Correction Factor (CCF
83
Applying the CCF
84
Automatic Calibration
84
Calibration Method
85
Non-Fluorescent Solutions
86
LIF Calibration for Different Dimension Capillaries
86
LIF Calibration for Different Fluorescent Solutions
87
Standard System Performance Test
87
Troubleshooting
88
APPENDIX B: Evaluating PA 800 Plus UV Filters
91
Overview
91
Other manuals for Beckman Coulter PA 800 plus
Application Guide
38 pages
Pre-Installation Guide
28 pages
4
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Beckman Coulter PA 800 plus Specifications
General
Brand
Beckman Coulter
Model
PA 800 plus
Category
Measuring Instruments
Language
English
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