Beckman Coulter PA 800 plus

Other manuals for Beckman Coulter PA 800 plus

Application Guide38 pages

Pre-Installation Guide28 pages

Questions and Answers

B
brownrachaelAug 12, 2025
What to do if I see low or unsteady current in my Beckman Coulter Laboratory Equipment?
- D
  Derrick MataAug 12, 2025
  If you're experiencing low or unsteady current with your Beckman Coulter Laboratory Equipment, it might be due to a plugged capillary. Try rinsing the capillary with DDI water at 100 psi for 10 minutes, and then perform the capillary conditioning method. Another potential cause is air bubbles in the gel. In that case, degas SDS-MW Gel Buffer under 5 to 15 Hg vacuum for 5 minutes.
J
John DavisAug 15, 2025
How to fix unsteady current after rinsing the capillary in Beckman Coulter Laboratory Equipment?
- S
  Shawn CookAug 15, 2025
  If rinsing the capillary doesn't solve the unsteady current issue in your Beckman Coulter Laboratory Equipment, the capillary may need to be replaced.
T
Trevor FordAug 19, 2025
Why are there no peaks on my Beckman Coulter PA 800 plus Measuring Instruments?
- D
  Daniel JonesAug 20, 2025
  If you are not seeing any peaks, consider the following possibilities: Ensure your LIF detector is calibrated by referring to the LIF calibration section. Verify that your laser is turned on. Check the probe stabilizer connection to the lock-down bar. Confirm the voltage is properly set in the method, ensuring the correct polarity (orange LED should be on). Check for bubbles in the sample vial. Inspect the capillary window for damage, and if broken, replace the capillary and clean the probe aligner with a water-moistened cotton swab.
A
Amy GibsonAug 23, 2025
What to do if I have low intensity peaks on Beckman Coulter Measuring Instruments?
- L
  Laura WoodAug 25, 2025
  Low intensity peaks can occur if the labeling reaction was not carried out properly. To ensure a successful labeling reaction: * Make sure that the protein is in a non-tris buffer, as Tris will compete with your carbohydrate. * Ensure that the carbohydrate pellets were fully dried, as any water residue will interfere with the labeling reaction yield. Also, enzyme activity may vary, so adjust the amount if the reaction yield is lower than expected.
T
Teresa MartinezAug 27, 2025
What to do if there is a shift in migration time between runs on Beckman Coulter PA 800 plus?
- K
  Kathleen HurstAug 27, 2025
  Migration time shifts can be associated with the length of injection. Ensure that injections are between 3 and 20 seconds at 0.5 psi, adjusting the injection time based on concentration.
C
Crystal LewisAug 30, 2025
How to fix low current in Beckman Coulter PA 800 plus Measuring Instruments?
- M
  Michelle GarciaAug 31, 2025
  To address low current issues: * Use approximately 50 cm N-CHO capillary total length (50 ?m I.D.) for a predicted current that should be steady at around -15 ?A. * If the capillary may be blocked, use Direct Control to perform a 70 psi rinse with water to unblock it.
A
Anthony FryeSep 3, 2025
What causes spikes in electropherogram in Beckman Coulter PA 800 plus and how to resolve it?
- J
  jesuswashingtonSep 4, 2025
  Spikes in the electropherogram can be caused by air dissolved in the gel buffer. To resolve this, sonicate the gel for 5 minutes.
A
allison77Sep 7, 2025
Why are there extra peaks in my Beckman Coulter PA 800 plus?
- C
  Charles MedinaSep 7, 2025
  Extra peaks can be caused by contaminants left over from the manufacturing process of vials. It is very important to use extra clean 0.5 mL micro-centrifuge vials, especially during the labeling step.

Summary

Revision History

Safety Notices

Symbols and Labels Overview

Describes symbols and labels used on the instrument and in the manual.

Hazard Symbols and Labels

Covers biohazard, moving parts, high voltage, laser, and sharp object warnings.

Regulatory and Disposal Labels

Information on recycling, mercury disposal, and RoHS compliance labels.

Alert Definitions

Defines the meaning of different alert types (Warning, Caution, Important, Note).

Installation Procedures

Introduction to Installation

Overview of installation procedures for detectors, lasers, and cartridges.

Detector Installation

Steps for installing UV, PDA, and LIF detectors into the instrument.

Detector Calibration

Procedures for calibrating PDA and LIF detectors after installation.

Filter Installation

Guides for installing wavelength filters for UV and LIF detectors.

Capillary Cartridge Management

Instructions for rebuilding and installing capillary cartridges.

Sample Vial Preparation

Guidelines for filling universal and micro vials for samples.

Maintenance Procedures

Maintenance Overview

Overview of maintenance tasks for the PA 800 plus hardware.

Interface Block Components Maintenance

Procedures for maintaining levers, electrodes, and the interface block.

Coolant System Maintenance

Procedure for filling and maintaining the instrument coolant.

Fiber Optics Maintenance

Cleaning and maintenance procedures for fiber optic cables.

Lamp Replacement

Procedure for replacing the deuterium lamp.

Instrument Care and Component Replacement

General care, LIF maintenance, quad-rings, and fuse replacement.

Interfacing PA 800 plus with Mass Spectrometry

CE-MS;MS Mode Cartridge Procedures

Procedures for using CE-MS/MS mode with standard or EDA cartridges.

Install Capillary in Standard Cartridge

Steps to install a capillary in the standard CE-MS/MS cartridge.

Install Capillary in EDA-Only Cartridge

Steps to install a capillary in the EDA-only CE-MS/MS cartridge.

LIF Test Mix Calibration and Performance Tests

LIF Calibration Concepts

Understanding RFU and Calibration Correction Factor (CCF).

Automatic Calibration Procedure

Step-by-step guide for performing automatic LIF detector calibration.

Calibration Method Details

Explanation of the simple method used for automatic calibration.

Performance Testing and Troubleshooting

Standard performance test and troubleshooting steps for LIF systems.

Evaluating PA 800 plus UV Filters

UV Filter Testing Overview

Introduction to testing UV filters for wavelength accuracy.

Filter Holder Tool Use

Description and use of the filter holder tool for spectrophotometers.

Detector Diagnostic Screen

Explanation of the PDA detector diagnostic screen for calibration.

Beckman Coulter PA 800 plus Specifications

General

Type	Capillary Electrophoresis System
Technology	Capillary Electrophoresis
Wavelength Range	190-600 nm (UV/Vis)
Capillary Length Range	10-100 cm
Voltage Range	0-30 kV
Temperature Control Range	15-60 °C
Data Analysis Software	32 Karat Software
Application	Pharmaceutical Analysis
Detection Method	UV/Vis
Capillary Diameter Range	20-100 µm
Detection Modes	fluorescence
Automation	Automated sample introduction and analysis