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PA 800 plus
Beckman Coulter PA 800 plus Application Guide
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A51969AA
April 2009
Beckman Coulter, Inc.
4300 N. Harbor Blvd.
Fullerton, CA 92835
Application Guide
P
A 800
plus
Pharmaceutical Analysis
System
Carbohydrate Labeling and Analysis
2
Table of Contents
Revision History
3
Safety Notices
5
Symbols and Labels
5
Introduction
5
Caution, Biohazard Label
5
Caution, Moving Parts Label
6
High Voltage Electric Shock Risk Symbol
6
Sharp Object Label
6
Recycling Label
7
Disposal of Devices Containing Mercury Components
7
Restriction of Hazardous Substances (Rohs) Labels
8
Rohs Caution Label
8
Rohs Environmental Label
8
Alerts for Warning, Caution, Important, and Note
9
Table of Contents
11
Carbohydrate Labeling and Analysis Kit
13
Overview
13
Safety Information
13
General Precautions
14
Materials and Reagents
14
Contents of this Kit
14
Materials Needed but Not Provided in Kit
14
Storage Conditions
15
Overview of the Procedure
15
Releasing Oligosaccharides from Glycoproteins
15
Enzymatic Release of the N-Linked Oligosaccharides
16
Working with the Supernatant
16
Principle of the Labeling Method
16
Preparing the Labeling Reagents
17
Choosing the Proper Fluorophore
17
Preparing the Reagents
17
Preparing the Standards
18
Performing the Labeling Reaction
18
Preparing the PA 800 Instrument
20
Installing and Pre-Conditioning the N-CHO Capillary
21
Clean the Interface Block
22
Insert the Cartridge
22
Preparing the Buffer Trays
22
Sample Vial Setup
24
Running the Samples
25
Initial Conditions
25
LIF Detector Initial Conditions
26
Time Program
27
Evaluation of Results
29
System Shutdown and Capillary Storage
30
Short Term Storage (<24 Hours) of Capillary
30
Long Term Storage (>24 Hours) of Capillary
30
Troubleshooting Procedures
31
Appendix
32
LIF Detector Calibration Procedure
32
Setting the Calibration Corrector Factors (Ccfs)
32
Automatic Calibration
33
CCF Troubleshooting Procedures
36
Ccf <1
36
No Step Change Detected
36
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Beckman Coulter PA 800 plus Specifications
General
Brand
Beckman Coulter
Model
PA 800 plus
Category
Measuring Instruments
Language
English
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