A51969AA
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Carbohydrate Labeling and Analysis Kit
Preparing the Labeling Reagents
Figure 1 Labeling Reaction of an Oligosaccharide with APTS
The efficiency of the labeling reaction is dependent on temperature and the amount of
oligosaccharides. This protocol has been optimized for labeling 5 nmoles or less of total
oligosaccharides.
Samples with amounts higher than 5 nmoles may give a lower reaction yield. Use maltose (G22) as
an internal labeling control and/or as an internal mobility marker.
Preparing the Labeling Reagents
Choosing the Proper Fluorophore
APTS-M is a high-purity fluorophore intended for labeling monosaccharides. APTS-M contains
citric acid as a catalyst. APTS (L6) can be used for oligosaccharides.
Preparing the Reagents
1
Prepare the APTS labeling reagent.
To a vial of APTS labeling dye (L6)
a. Add 48 μL of Labeling dye solvent (15% acetic acid – L3).
b. Vortex for 5 seconds until complete dissolution.
c. Store at -35 to -15°C for up to 2 weeks when not in use.
2
Prepare the APTS-M (Monosaccharide grade) Labeling Dye
a. Add 400 μL of DDI water to the APTS-M vial.
b. Vortex the mixture for 5 seconds until all of the solid is dissolved.
c. Store the reconstituted APTS-M at -35 to -15°C for up to two weeks.
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