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Bio-Tek ELx405 - Performance Qualification (PQ)

Bio-Tek ELx405
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Rinse/Soak the Fluid Path | 113
3 Connect a bottle containing four liters of deionized water and run
the
P_DAY_RINSE Prime program three times to flush the system.
Using Ethanol:
At the end of the day:
1 Prepare a four-liter solution of 10% ethanol and deionized water.
(
Do not use isopropyl alcohol.) Connect the bottle to the washer
(Valve “A” if the valve module is connected).
2 Run the DECONTAMINATION Maintenance program.
3 Connect a bottle containing four liters of deionized water and flush
the system by running either the
P_DAY_RINSE Prime program
(three times), or the
OVERNIGHT_LOOP Maintenance program.
Using Deionized Water:
If the washer will be idle between plates for longer than 45 minutes, flush
the proteins from the washer:
1 Fill a supply bottle with deionized water. Connect the bottle to the
washer (Valve “A” if the valve module is connected).
2 Run the P_DAY_RINSE Prime program.
3 Enable AUTOPRIME for 60-minute intervals.
At the end of the day:
1 Fill a supply bottle with deionized water. Connect the bottle to the
washer (Valve “A” if the valve module is connected).
2 Run the P_DAY_RINSE Prime program three times.
3 Run the OVERNIGHT_LOOP Maintenance program.
Weekly/Monthly
1 Flush the system with 0.1-0.5 N NaOH, followed by neutralization
with an equivalent normality (0.1-0.5 N) of HCl.
2 Rinse well with deionized water to remove the HCl.
3 Run the OVERNIGHT_LOOP Maintenance program with deionized
water, or run the
P_DAY_RINSE Prime program three times with
deionized water if you plan to use the washer immediately.
ELx405 Operator's Guide

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