250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 9
Pulmonary cytodiagnosis: the mucous in sputum can be evenly dispersed
giving a quick representative sample of calls for cytologic examination. Cells are
liberated from the mucous of sputum that had been immersed in 50% alcohol or a
fixative.
Ragweed pollen: 15 ml dispersion completely disintegrates in 11 minutes.
Rat bone: 1/2 gm disintegrates in 4 minutes.
Rat liver: complete disruption in 3 minutes.
Rat liver mitochondria: ultrasonic treatment has been used extensively for
the varied research performed on this material. Disruption occurs in seconds.
Rat skin: 1 gm completely disintegrates in 4 minutes.
Red and white blood cells: ultrasonic treatment breaks particle size to 100
Angstroms. Complete disruption in 1 minute. 25 gms/100 ml, saline or plasma,
sample treated 15 seconds, 35% disruption. Adenosine triphosphate was shown to
be membrane-bound by this method.
Reovirus: dissociates cell-bound and aggregated virus. Maximum titer with 4
ml of virus was achieved in 2 minutes.
Retinal outer segments: ultrasonic treatment breaks particles down to almost
molecular size.
Rhodopseudomonis palustris: complete disruption in 4 minutes.
Rhodospirillum rubrum: excellent disruption in a few seconds.
Rimosus: Monocellular elements from surface-grown colonies obtained in 1
minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
RNA: rapid and thorough re-suspension of 9 TCA pellets during extractions.
Rocks: excellent for disaggregation of sedimentary rock and for cleaning mate-
rial rock surfaces between polishing stages.
S. faecalis: excellent disruption in 1 minute.
S. fragilis: 5 minutes yielded excellent release of galactokinase, more than any
other method. Subcellular particles may be extracted or disrupted.
Saliva glands: complete disruption.
Salmonella: various culture media or phosphate buffered saline disintegrated
between 40 and 50% in 10-20 minutes. Sonifying was one step in an improved
assay for enzyme thiogalactosize transacetylase.
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