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Branson Sonifier 250 User Manual

Branson Sonifier 250
82 pages
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250 - 450 Sonifier
Analog Cell Disruptor
User’s Manual
EDP 100-413-016
Rev. C
BRANSON Ultrasonics Corporation
41 Eagle Road
Danbury, Connecticut 06813-1961 U.S.A.
(203) 796-0400
http://www.sonifier.com
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Branson Sonifier 250 Specifications

General IconGeneral
Power Output250 watts
Frequency20 kHz
Amplitude Range0-100%
Timer1 second to 15 minutes
Voltage115/230 V, 50/60 Hz

Summary

Manual Change Information

Copyright Notice

Chapter 1: Safety and Support

Safety Considerations

Crucial safety precautions to observe when operating the Analog Sonifier.

About this Manual

Explains signal words like WARNING, CAUTION, NOTICE.

Intended Use of the System

Lists applications and power differences between 250W and 450W Sonifiers.

Regulatory Compliance

Details compliance standards for Sonifiers sold in Europe.

Product Warranty Information

Outlines the terms, conditions, and exceptions of the product warranty.

How to Contact Branson

Provides contact information for customer service, technical support, and parts.

Preparing for Support Calls

Lists information to prepare before contacting Branson for support.

Returning Equipment for Repair

Details the procedure and requirements for returning equipment for repair.

Obtaining Replacement Parts

Explains how to contact the Parts Store and what information is needed.

Chapter 2: Introduction

Main Components Overview

Identifies the primary components of the Sonifier unit.

Principle of Operation

Explains how the Sonifier converts electrical energy into mechanical vibrations.

Power Supply Front Panel

Details the devices and functions on the front panel of the power supply.

Power Supply Rear Panel

Describes the components and connections on the rear panel.

Chapter 3: Unpacking and Handling

Unpacking and Handling Procedures

Instructions for unpacking the unit and inspecting for shipping damage.

Chapter 4: Installation and Setup

Equipment Placement Guidelines

Guidance on selecting a suitable location for the Sonifier unit.

Power Requirements

Specifies the required single-phase voltage and frequency for operation.

Setup Procedure

Step-by-step guide for setting up the converter/horn assembly.

Equipment Testing

Procedure for establishing a baseline for future equipment testing.

Connecting and Removing Components

Instructions for attaching and detaching horns and tips.

Connecting Horn to Converter

Details on how to correctly connect the horn to the converter.

Connecting Tip to Horn

Steps for attaching a tip to the horn, including torque specifications.

Guards and Safety Equipment

Information on necessary guards and personal protective equipment.

Chapter 5: Technical Specifications

General Specifications

Details electrical, mechanical, and dimensional specifications for the Sonifier.

Electrical Specifications

Lists electrical specs like voltage, frequency, power, fuse, leakage.

Mechanical Specifications

Provides weight and dimensions for converter and power supply.

Unit Dimensions

Shows side and front view dimensions of the Sonifier unit.

Chapter 6: Operation

General Operation Modes

Explains basic operation modes: Timed, Continuous, and Pulsed.

Timed Operation

Describes setting the timer for specific processing durations.

Continuous Operation

Details how to set the unit for continuous operation.

Pulsed Operation

Explains the pulsed mode and duty cycle control for temperature management.

Operating Considerations

Discusses techniques for optimal ultrasonic processing and temperature control.

Limiting Temperature Rise

Covers methods to manage sample temperature during operation.

Vessel Capacity and Temperature Rise

Relates vessel size to cooling requirements and temperature rise.

Minimizing Undesirable Factors

Addresses issues like foaming, aerosoling, and discoloration.

Sterilizing and Preventing Cross-Contamination

Instructions for sterilizing horns and tips.

Disrupting Tissues and Solids

Techniques for processing biological tissues and solid materials.

Using Glass Powders with Solution

How to use glass powders to enhance cell disruption.

Horn and Micro Tip Amplitudes

Details amplitudes for various horn and micro tips.

Chapter 7: Maintenance

General Maintenance Information

Directs users to contact support for maintenance needs.

Maintenance and Troubleshooting

Covers periodic inspection and maintenance of tooling components.

Tip Erosion

Explains tip erosion, its causes, and signs of wear.

Power Output Loss

Discusses causes of power loss and initial troubleshooting steps.

Preventive Maintenance

Outlines periodic cleaning of the equipment.

Reconditioning the Stack Interface

Describes how to maintain the converter-horn mating surfaces.

Refacing Mating Surfaces

Details the process of cleaning and reconditioning mating surfaces.

Stud Replacement

Instructions for replacing the stud on the horn/converter assembly.

Horn Tip Cleaning

Steps for cleaning the threads of the horn tip.

System Trouble Analysis Chart

A chart to diagnose symptoms and probable causes of system issues.

Appendix A: Applications

Actinomyces

3 minutes of sonifying produced excellent disruption with 50% protein released.

Aerobacter aerogenes

Excellent breakage with better enzyme release than other methods.

Algae secendesmus

10 ml concentrated solution completely disrupts in 1 minute.

Alkaloids

Extraction from ipecac root yielded more alkaloid than Soxhlet extraction.

Antibioticus

Monocellular elements from surface-grown colonies obtained in 1 minute.

Antigen

Used extensively to produce antigens and vaccines, increasing yield.

Aorta

1 gram disintegrates in 2 minutes.

Ascaris eggs

8 ml concentrated solution completely disrupts in 4 minutes.

Appendix B: Parts List

Replacement Parts

Lists replacement parts like fuses, washers, studs, converters, and wrenches.

Appendix C: Accessories

Micro Tip

Useful for processing small volumes, available in tapered and double-stepped designs.

Cup Horn

Allows treatment in small tubes without immersing the horn, ensuring sterile conditions.

Flow-thru Horn

Designed for pharmaceutical research, allows continuous processing or mixing.

Rosette Cell

Conical glass cell for exposing substances to ultrasonic energy during circulation.

Continuous-Flow Attachment

Allows continuous processing of low-viscosity materials for emulsifying/dispersing.

Soundproof Box

Reduces mechanical noise generated during ultrasonic processing.

Appendix D: Accessories Parts List

D.1 Accessories Parts List

Lists disruptor horns and their EDP numbers.

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