Appendix C Accessories
C - 2 100-413-016 Rev. C
The double-stepped micro tip is a two-piece horn, consisting of a coupling section
and a lower tip. Because the coupling section is attached directly to the converter,
the standard disruptor horn must be removed prior to using the double-stepped
micro tip. Recommended for use on extremely small volumes, the double-stepped
tip can be used to treat volumes ranging from 0.5 to 20 ml. Applications for this
tip include red and white blood cells, tissue culture cells, HeLa cells and the com-
plete range of cells which have low to medium resistance to breakage.
To prevent foaming or aerosoling while processing small quantities with the
tapered or stepped micro tips, the use of a conical shaped tube such as an eppen-
dorf tube or a cut down centrifuge tube is recommended.
!
When using micro tips, a maximum Output Control Setting of “6” must
not be exceeded. The micro tip will break if driven at higher amplitudes.
C.2 Cup Horn
Cup Horns allow materials to be treated in small eppendorf style tubes or test
tubes without immersing the ultrasonic horn or micro tip in the material, thereby
providing completely sterile conditions. The Cup Horn is attached directly to the
converter and the assembly is mounted upside down on the lab stand, or in our
Sound Enclosure with the assembly inserted through the bottom. With chilled
water in the Cup Horn, the tubes are suspended in the Cup with the contents of the
tubes just below the water level. Ultrasonic energy is then conducted from the sur-
face of the horn, through the water and tube walls, to the contents of the tube.
Some energy loss may be experienced when applying ultrasonics in this indirect
way and processing may take longer than if the ultrasonic horn were immersed
directly in the solution.
There are three types of Cup Horns, a high intensity unit that will accommodate a
single test tube, and two larger units for multiple test tubes. The high intensity Cup
Horn has a concave bottom that focuses the ultrasonic energy on the bottom of the
tube. The larger units, with diameters of 2 or 3 inches, allow for the immersion of
multiple test tubes. The larger Cup Horns have clear plastic walls, which permit
easy viewing of the activity in the tubes during processing. Both types of Cup
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