250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 1
Appendix A Applications
Actinomyces: 3 minutes of sonifying produced excellent disruption with 50%
protein released and excellent enzyme activity.
Actinomycin D: suspended in 3 minutes.
Aerobacter aerogenes: excellent breakage with better enzyme release than
any other method. A low power setting can release sulfatase activity into the
supernate with no obvious disruption of the majority of cells.
Aerobacter suboxydans: excellent breakage but requires higher power than
A. aerogenes.
Algae secendesmus: 10 ml concentrated solution completely disrupts in 1
minute.
Alkaloids: total amount and speed of extraction are greater using the Sonifier
Cell Disruptor than with standard methods. Extraction from ipecac root in 30 sec-
onds yielded more alkaloid than Soxhlet extraction in 5 hours.
Antibioticus: monocellular elements from surface-grown colonies obtained in
1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Antigen: the Sonifier Cell Disruptor is used extensively to produce antigens and
vaccines. It can increase yield or expose otherwise unobtainable sites.
Aorta: 1 gram disintegrates in 2 minutes.
Aphanomyces: after blending, complete disruption in 3 minutes.
Arthobacter tumescens: 10 gm in 40 ml in 5 minutes for O coumaric reduc-
tose.
Ascaris eggs: 8 ml concentrated solution completely disrupts in 4 minutes.
Asperigillus: complete disruption in 4 minutes.
Aurefaciens: monocellular elements from surface-grown colonies obtained in
1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
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