Appendix A Applications
A - 2 100-413-016 Rev. C
Azotobacter vinelandii: 15 ml buffered solution, 200 mg wet wt. per ml com-
pletely disintegrates in 2 minutes.
B. anthracis: 80% disruption of anthracis in 4 minutes. Complete disruption of
10 ml of eryisipelothrix rhusipathiae in 10 minutes.
B. cereus veg cells: disruption in a few seconds.
B. cereus spores: disruption of 10 mg/6 ml in 13 minutes.
B. megaterium spores: complete breakage of a concentrated 6 ml solution in
15 minutes.
B. sphaericus: major disruption in 1-3 minutes.
B. stereothermophilis spores: complete disruption in 2 minutes.
B. subtilis: disruption of 5 gm wet wt, 15 ml buffer, in 5 minutes.
B. subtilis veg cells: heavy suspension clears in 1 minute.
Bacillus: stereothermophulus (thermophillic spore form). 98% disruption of 70
ml of 40% suspension in 15 minutes.
Bacillus brevis: 1:15 W/V in 3 minutes.
Bacteroides Symbiosis: 1-phosphofructokinase a soluble enzyme has been
isolated from this anaerobe by ultrasonic treatment. A 25 ml suspension was son-
ified for 10 minutes and centrifuged at 36,000 xg for 10 minutes.
Baker’s yeast (saccharomyces cerevisiae): see Yeast.
Blastomyces dermatitidis: 95% disruption in 3 minutes.
Blood cells: red and white cells can be disrupted in a few seconds.
Boll weevil tissue: complete homogenization in a few seconds.
Bone: compact bone can be sonified and processed for microscopic sections in
minutes. Other methods can require up to a week. Bone specimens treated in this
way yielded large numbers of intact cells with little distortion. Malignant criteria
were easily recognized. Tumor types studied were: osteosarcoma, chondrosar-
coma, liposarcoma, chordoma, metastic bronchogenic squamous and benign
giant. Bone can be decalcified without injury to the cells, processed for micro-
scopic sections and diagnosed in a short time. Other methods require extensive
treatment time.
Brain stem and adrenal gland: ultrasonic treatment dispersed 10 mg sam-
ples in 10 ml fluid which is usually difficult without substantial loss of material.
The suspension was analyzed for nucleotides.
Brain tissue: disintegrates instantly.
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