250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 3
Brevi bacterium: 25 ml disrupts in 20 seconds.
Brevi bacterium acetylicum: approximately 3 minutes to disrupt large sam-
ples and measure TCA enzyme activity.
Brine Shrimp: complete disintegration in 1 minute.
Brucella abortes: separates easily from leukocytes. At least 9 antigens
extracted.
Bull sperm: contractile protein is easier to extract from tails after sonifying.
C. butyricum, C. cylinrosporum, C. kluyveri: vegetative cells easily dis-
rupted.
C. pasteurianum: 3 minutes disruption for hydrogens reducing Ferredoxin
with H2.
Calcium: mouse Ehrlich ascites tumor cells were sonified for 1 minute to deter-
mine the amount of bound calcium present. Cells were labelled with calcium 45.
Candida albicans spores: 95% disruption of 1/2 gram dry wt. in a 15 ml solu-
tion in 35 minutes.
Carbon black: excellent small particle suspension.
Caryophanon latum: glucosamine, muramic acid, alanine, glutamic acid and
Iysine were obtained.
Catecholamine:
can be extracted from heart muscle.
Cellumonas biazotea:
disruption obtained with retention of malate dehydrogenase activity. Chicken
spermatozoa: complete disrupts in 2 minutes.
Chlorella:
completely disrupts in 3 minutes.
Chloroplasts: disrupts in a few seconds.
Cholesterol: apparent permanent suspension in 1 minute in water.
Chromatography: prior ultrasonic treatment of adsorbant in any convenient
solvent for a few seconds eliminates aggregates and results in a uniform, easily
packed column.
Clostridium: quickly disrupts all types.
Coagulase-globulin: ultrasonic treatment before precipitation yields signifi-
cantly more enzyme.
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