250 - 450 Sonifier Appendix A Applications
Instruction Manual
100-413-016 Rev. C A - 11
Streptomyces: monocellular elements from surface-grown colonies obtained
in 1 minute. Complete disruption in 5 minutes, 50% disruption in 2 minutes.
Subcellular particles: may be separated or broken depending on power selec-
tion and length of time.
Sulfanilamide: excellent dispersion in less than 1 minute. Continued ultrasonic
treatment will produce complete disruption.
Sulfapyridine: excellent dispersion in less than 1 minute. Continued ultrasonic
treatment will produce complete disruption.
Synovial fluid: ultrasonic treatment is an excellent means of reducing fluid vis-
cosity. It is simpler and faster than using hyaluronidase.
T. pyriformis: excellent disruption, 8 enzymes released.
Tablets: complete disruption in 2-40 seconds, depending on type.
Tea: excellent extraction.
Tetrahymena: disrupts in a few seconds. Enzymes which have been monitored
include: succinate, lactate, B-hydroxy butyrate, glutamate and DPNH oxidases,
DPNH-cytochrome C reductase and ribonuclease. Activity of DPNH oxidase was
twice that of the best previous experiments.
Thermoactinomyces: disruption of hyphae. Homogenization of protein com-
plex without denaturation.
Thermophile negative: good disruption within 2 minutes.
Thermophilic bacillus: Isocitrate lyase was extracted from a spore-forming
bacillus similar to Stearothermophilus. A washed cell paste suspended in a phos-
phate buffer was sonified 1-2 minutes and the supernatent was used for enzyme
experiments without further treatment. Extracts could be frozen and stored with-
out loss of activity.
Thiouric acid: dissolved in a few seconds.
Thymus cells: complete disruption in 15 seconds.
Tissue culture cells: complete disruption in a few seconds. To avoid damage
to free organelles and to obtain intact Lyososomes, use low power at short expo-
sure.
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