Click Calibration… Calibrate camera orientation on the menu bar.
You will see your reference image; where data is present, the image is overlaid with blue. Click on one of your
measurement marks, and then the other. You will be asked to enter the distance between these points to establish scale:
Enter the previously measured distance. If an incorrect distance is entered, the calibration will succeed, and measurements
will be successful; however, the scale of all your future results will be off by a constant factor.
Click Calculate, and a score will be displayed.
If the score is acceptably low (i.e., less than 0.05 pixels), click Accept to finish. If the score is high, there is a problem with
the source data; check your area of interest and initial guess and check the sigma variable in the output data to eliminate
any problem areas.