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cytiva Biacore 8K User Manual

cytiva Biacore 8K
106 pages
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Biacore 8K/8K
+
Operating Instructions
Original instructions
cytiva.com
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cytiva Biacore 8K Specifications

General IconGeneral
SoftwareBiacore Insight Evaluation Software
Sample ThroughputUp to 96 samples per run
Number of Flow Cells8
Data Analysis SoftwareBiacore Insight Evaluation Software
System TypeSurface Plasmon Resonance (SPR) system
Flow Cell8 flow cells
Temperature Control4-40°C
Flow Rate Range1-100 μL/min
ApplicationsKinetics, affinity, concentration

Summary

Introduction

About this manual

Explains the purpose, scope, and typographical conventions used in the manual.

Important User Information

Details critical user information, intended use, and prerequisites for safe operation.

Safety notices

Defines and explains safety notices like WARNING, CAUTION, and NOTICE.

Notes and tips

Provides information on notes and tips for optimal product usage and troubleshooting.

Associated Documentation

Lists and describes user documentation delivered with the product and available online.

Glossary

Explains Biacore terminology used in the manual.

Safety instructions

Safety Precautions

Details general, flammable liquids, personal protection, installation, power, operation, maintenance, and decommissioning precautions.

Labels

Describes various labels found on the product and their meaning.

Safety Labels and Other Symbols

Details safety labels and other symbols attached to the instrument.

Emergency Procedures

Describes how to perform emergency stops and procedures in case of power failure.

System description

Instrument Components

Identifies the main parts of the Biacore 8K and 8K⁺ instruments with diagrams and labels.

Flow System

Describes the liquid supply, tubing inlet panel, buffer selector, pumps, and flow path.

Temperature Control

Explains flow cell, sample compartment, and sample hotel temperature settings and their impact.

Sample Handling

Details microplate preparation, covers, trays, and sample injection procedures.

Signal Detection and Processing

Describes the sensor chip, surface plasmon resonance (SPR), and SPR response data.

System Software

Explains the Control Software, Evaluation Software, and software notifications.

Installation

Site Requirements

Details space, environmental, electrical, and computer requirements for installation.

Unpacking, Assembly, and Transport

Covers unpacking, assembly, lifting, moving, and transport precautions for the instrument.

Reinstalling the Software

Provides steps for downloading and installing or repairing the system software.

Connections

Explains how to connect the instrument to mains power and to the computer.

Operation

Starting the System

Details steps to start the instrument and Control Software, including license and database setup.

Preparing for a Run

Covers setting temperature, liquid supply, sensor chip, and methods for a run.

Preparing and Loading Samples

Guides on preparing samples/reagents and loading microplates onto sample trays.

Performing the Run

Explains how to start, monitor, and clean up after a run, and enter standby mode.

Closing Down the Software

Describes options for shutting down the Control Software based on system state.

Maintenance

User Maintenance Operations

Details regular maintenance schedule, required materials, and cleaning procedures.

Storage

Describes the conditions for storing the instrument for long periods.

Troubleshooting

Troubleshooting Tools

Explains notifications and how to perform a system check for troubleshooting.

Instrument Problems

Lists common instrument problems and their possible causes and corrective actions.

Reference information

System Specifications

Provides general specifications, environmental conditions, and operational parameters for the instrument.

Ordering Information

Provides information on how to order the Biacore system and related products.

Chemical Resistance of Wetted Parts

Lists compatibility of flow system components with various solutions and concentrations.

Recycling Information

Contains information on decontamination, decommissioning, and disposal of the product and its components.

Contact Information

Provides contact details for support and troubleshooting reports.

European Union and European Economic Area

Describes regulatory information and CE marking for the EU and EEA.

Eurasian Economic Union

Details information applicable to the product in the Eurasian Economic Union.

Regulations for North America

Describes information applicable to the product in the USA and Canada, including FCC compliance.

Regulatory Statements

Presents regulatory statements applicable to regional requirements, including South Korea.

Other Regulations and Standards

Describes standards that apply to the product and regulatory compliance of connected equipment.

Declaration of Hazardous Substances (DoHS)

Provides product pollution control information as per Chinese standards.

Health and Safety Declaration Form

Provides a form for on-site service to declare equipment safety and cleanliness.

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