cytiva BPG 450 Columns

Questions and Answers

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Jo OconnorAug 9, 2025
How to fix high-back pressure in Cytiva Laboratory Equipment?
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  Kayla SuttonAug 9, 2025
  High back pressure in Cytiva Laboratory Equipment can occur if the valves between the pump and the collection vessel are closed; in this case, open the valves. It can also happen if the valves are blocked, so you should check that all valves are clean and free from internal blockage. Other potential causes include the medium being packed too hard (repack the medium), blocked column nets (change nets), a blocked support net (clean or change the support net), an incorrect flow valve (change flow valve), or an incorrectly selected flow cell (select adequate size of flow cell). Also, check to see if the sample and collection vessels are at approximately the same level as, or above, the pump.
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Sharon JenningsAug 15, 2025
How to remove air trapped in the cytiva BPG 450 Columns Laboratory Equipment column?
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  Erica BeanAug 16, 2025
  Air trapped in the Cytiva Laboratory Equipment column can occur for several reasons. The buffers might not have the same temperature as the column, so check the buffers are at the same temperature as the column. The connections might not be correctly fitted together, so check the connections. The valves might not be shut tight enough, so check the valves and close them. The volume of the trap might be too small or large; in this case, fill air trap with buffer or change air trap.
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jacobsonsierraAug 21, 2025
What to do if the chromatogram is not as expected in cytiva BPG 450 Columns?
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  Johnny FitzgeraldAug 21, 2025
  If you're experiencing a chromatogram that's not as expected with your Cytiva Laboratory Equipment, several factors could be at play. The linear velocity might not be as expected, so check the sample. Back-mixing might have occurred anywhere in the column set-up, so check connections. The sample could have passed through an air trap, so check connections or test method. The adapter might not be correctly positioned in close contact with the bed; in this case, move the adaptor down 5 mm into the bed, or disassemble and assemble the column. Also, check the column packing, equilibrate the column, and check the sample and sample handling to ensure old sample hasn't mixed with new.
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Morgan TaylorAug 27, 2025
Why is my cytiva BPG 450 Columns Laboratory Equipment column leaking?
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  Jasmine JohnsonAug 27, 2025
  Column leakage in Cytiva Laboratory Equipment can be caused by several factors. The end-piece and/or flange might not be properly positioned with respect to the tube; in this case, disassemble and re-assemble the column. The solvents used might not be compatible with the O-rings and gaskets, so check solvent. The tubing might not be compatible with all the solvents, so change solvent. The connectors might not have the same dimensions, so change connectors. Air might be leaking into the column from the suction side of the pump, so check that the connections to and from the pump are correct and tightened. The torque might not be correct on the rods or the flange, so check that the correct torque specification has been used during column assembly.
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Thomas DixonSep 2, 2025
What to do if cytiva BPG 450 Columns column is infected and/or pyrogen contaminated?
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  Richard RodriguezSep 2, 2025
  If your Cytiva Laboratory Equipment column is infected or contaminated with pyrogens, it could be due to several reasons: the column bed might show signs of bacterial growth, so you should perform CIP and prepare new buffers/sample. The starting material might be contaminated, so use clean material. The in going components (such as water, serum components in growth media, etc.) might not be ok, so check material. If positive LAL tests were seen after a CIP cycle with NaOH, it might be because the NaOH has been removed, so repeat cleaning.

Summary

Introduction

Important user information

Provides critical safety and operational guidelines for users.

Safety instructions

Safety precautions

Details essential safety measures for operating the equipment.

Emergency procedures

Outlines steps to take in case of an emergency situation.

Column descriptions

BPG 100, 140 and 200 columns

BPG 300 column

BPG 450 column

Operation

Preparation

Column flow packing method

Explains the two-step method for packing the column.

Slurry preparation

Guides on how to prepare the media slurry for column packing.

Column packing

Describes the steps involved in packing the BPG columns with media.

Performance evaluation of the column

Describes methods to assess the packed column's efficiency.

Maintenance

Service and preventive maintenance

Replacements of nets and O-rings

Explains how to replace worn nets and O-rings.

Disassemble and assemble the adapter

Provides instructions for taking apart and reassembling the adapter.

Leakage test

Describes the procedure for testing the column for leaks.

Cleaning

Troubleshooting

Air in the column

Addresses problems related to air trapped within the column.

cytiva BPG 450 Columns Specifications

General

Column Diameter	450 mm
Material	Borosilicate glass, stainless steel
Maximum Operating Pressure	3 bar (0.3 MPa, 43.5 psi)
Operating Temperature	4 to 40 °C
Temperature Range	4°C to 40°C
Chemical Resistance	Resistant to most common buffers and cleaning agents
Applications	protein purification