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cytiva OligoPilot 400 User Manual

cytiva OligoPilot 400
152 pages
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OligoPilot 400
O
perating Instructions
Original instructions
Translation disc included
cytiva.com

Table of Contents

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cytiva OligoPilot 400 Specifications

General IconGeneral
Number of Amidites4
Number of Channels4
AutomationFully automated
User InterfaceTouch screen
Flow rateAdjustable
Operating temperatureAmbient
Reagent ConsumptionOptimized reagent consumption
TypeAutomated oligonucleotide synthesizer

Summary

Introduction

Important User Information

Crucial information for safe operation, including warnings, intended use, and user prerequisites.

About This Manual

Explains the manual's purpose, scope, and typographical conventions for better understanding.

Associated Documentation

Lists related system and software documents for comprehensive technical information.

Safety Instructions

Safety Precautions

Detailed safety measures covering general precautions, explosion hazards, personal protection, and power.

Labels

Describes system labels and regulatory markings, including ATEX classification for safety.

Emergency Procedures

Covers emergency shutdown, power failure consequences, and system restart procedures.

System Description

Overview

Overview of the oligonucleotide synthesis process steps executed by the OligoPilot 400.

Illustrations of OligoPilot 400

Visual guide to system components and features with labeled diagrams for clarity.

System Components

Detailed overview of key system parts like pumps, valves, sensors, connections, and gas delivery.

Containers for Reagents and Solvents

Requirements for reagent bottles, including capacity, safety coatings, and leakage detection.

Columns

Information on recommended columns for use and specifications of the column holder.

Flowchart

Diagram illustrating the system flow path, showing component positions and tubing connections.

UNICORN Control System

Description of the UNICORN software used for controlling and supervising the OligoPilot 400.

Installation

Unpacking and Transport

Guidelines for unpacking, site requirements, and safe transport of the OligoPilot 400 equipment.

Setup

Step-by-step instructions for setting up the system, including cabinet adjustment and connections.

Installation Procedure

Procedures for installing UNICORN software, compressed air, and inert gas supply.

Operation

Prepare the System

Steps to prepare the OligoPilot 400 for operation, including safety precautions and system startup.

Column Packing Procedure

Guidelines for preparing solutions and a detailed procedure for packing the chromatography columns.

Perform a Run

Covers final checks, executing a synthesis run, and viewing/printing results via UNICORN.

Procedures After a Run

Instructions for post-run tasks, including unpacking columns and deprotection steps.

Maintenance

User Maintenance Schedule

Defines daily, weekly, monthly, and annual maintenance tasks for system upkeep and longevity.

Component Maintenance

Procedures for maintaining key components like pumps, membrane valve blocks, and PCV valves.

Cleaning

Instructions for cleaning the instrument, between runs and before planned service.

Storage

Guidance on preparing the OligoPilot 400 for both short-term and long-term storage.

Troubleshooting

Reference Information

Specifications

Technical data for the OligoPilot 400, including physical dimensions, power, and environmental requirements.

Chemical Resistance

Guidelines on the chemical resistance of system components to various substances used in the process.

Recommended Reagents

Lists recommended reagents and their concentrations, crucial for synthesis performance.

Recycling Information

Information regarding product decommissioning, decontamination, and proper disposal methods.

Regulatory Information

Details regulations and standards applicable to the product in various regions, including EMC and safety.

Health and Safety Declaration Form

Form for service personnel to declare the safety and cleanliness of the equipment before maintenance.

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