DLAB Laboratory Instruments DM0412S - Tube Cleaning and Sterilization

12
2) Notice
 The centrifuge rotor can separate samples with a density lower than 2.0g/ml. If the samples density is
over 2.0g/ml, please calculate allowable speed depending on the following formula.
)/density( ample
)g/（0.2
max)speed(llow
mlgs
ml
speedrpma 
● Calculate RCF
An RCF can be determined with the following calculation formula.
RCF=1.118×r×n
2
×10
-5
r—rotating radius, unit: cm; n—rotating speed, unit: rpm
3) Autoclaving
A12-10P rotor is made of plastic, cannot be high-pressure sterilization and UV irradiation, only ordinary
sterilization can be used.
10.2 Tubes
1) Cleaning and sterilizing tubes
O：Applicable X：Inapplicable
Conditions Materials
PA
PC
PP
Cleaning
Cleaning fluids
Acidic（pH5 or lower）
X
X
X
Acidic（higher than pH5 ）
O
O
O
Alkaline（higher than pH9 ）
O
X
O
Alkaline（pH9 or lower）
O
O
O
Neutral（pH7）
O
O
O
Warm water(up to 70℃)
O
O
O
Ultrasonic cleaning
Neutral detergent（pH7）
O
O
O
Sterilization
Autoclaving
115℃（0.7kg/cm
2
）30minutes
O
O
O
121℃（1.0kg/cm
2
）20 minutes
X
O
O
126℃（1.4kg/cm
2
）15 minutes
X
X
X
Boiling
15 to 30 minutes
O
O
O
Ultraviolet sterilization
200-300nm
X
X
X
Gas sterilization
Ethylene oxide
O
X
O
Formaldehyde
O
O
O
PA: Polyallomer PC: Polycarbonate PP: Polypropylene
Table 10.2 Cleaning and sterilizing conditions for tubes
1) Cleaning PC tubes
PC material is low in chemical resistance against alkaline solutions. Avoid using neutral detergents with
pH higher than 9. Note that pH of some neutral detergents are still higher than 9 even if diluted according