Eppendorf Concentrator plus - 5.3.5 Loading a swing-bucket rotor

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Operation
Concentrator plus/Vacufuge® plus
English (EN)
5.3.5 Loading a swing-bucket rotor
Prerequisites
•A combination of rotor, bucket and adapter, approved by Eppendorf.
• Two inserted buckets.
•Matching and tested tubes and plates.
•Adapters and plates with a total height of ≤ 27 mm
1. Check the bucket grooves for cleanliness and grease lightly with pivot grease (order no. int.: 5810
350.050 / North America: 022634330).
Dirty grooves and pivots prevent buckets from swinging out evenly.
2. Hang the buckets into the rotor.
3. Check that both buckets are hanging properly and can swing freely.
4. When using a plate type for the first time, carry out a manual loading and swing-out test.
5. Check maximum payload (adapter, plate and content) per bucket.
The relevant details can be found on the rotor and in this operating manual (see Rotors on p. 12).
6. Load the buckets symmetrically when inserting adapters and plates.
The plate arrangement shown on the right-hand side is incorrect, as the buckets will not swing properly.
The plates have some play in the buckets.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
 Load rotors symmetrically with identical tubes or plates and buckets.
 Always load all positions of a swing-bucket rotor with buckets.
 Only load adapters with suitable tubes or plates.
 Always use tubes or plates of the same type (weight, material/density and volume).
 Ensure that tubes that are located opposite each other contain liquids with the same rate of
evaporation. Otherwise an imbalance can occur and the concentration may automatically
stop.
 Check that loading is symmetrical by balancing the adapters and tubes or plates used with
scales.
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone. Check the loading, balance the tubes and re-start the
centrifugation.
NOTICE! Filling the plates too high can cause overflowing.
During the run the meniscuses in the tubes along the edges of the plates are at an angle. This
is due to the centrifugal forces and cannot be avoided.
 Fill the plate wells to a maximum of 2/3 of the maximum filling volume.