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GE akta start Maintenance

GE akta start
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ÄKTA start Maintenance Cue Card, 29-0240-43 AC 10
Cleaning the Fraction collector
Maintenance interval
Clean the Fraction collector when required, for example in
case of liquid spill in the Bowl assembly.
Required solutions
Water
20% ethanol
Cleaning cloth
Note: The inlet lters are mounted on the inlet tubing at the
ending that will be immersed in the buer solution.
Replace the inlet lters
Note: An Inlet Filter Set containing inlet lters and support
nets is included in the accessories kit.
Instruction
Pull o the inlet lter and the support net from the inlet
lter holder.
Fit the new support net and inlet lter, and press the
lter in position into the inlet lter holder.
1.
2.
Replace the tubing and connectors
Maintenance interval
Replace tubing and connectors when the tubing is
clogged or bent so that the ow is obstructed, or when
leaking.
Note: Before starting to replace tubing and connectors,
clean the system ow path with DM water, then remove
the inlet tubing from water.
Instruction
Unscrew the connectors, and disconnect the tubing.
If the tubing has labels, remove the labels and use
them with the new tubing later. Discard the old tubing
and connectors.
Cut the new tubing to the same length as the original
tubing. Use a tubing cutter to get a straight angle cut.
Mount the connectors on the tubing.
Insert the tubing with connector into the port. Make
sure to insert the tubing all the way into the bottom of
the port.
Tighten the connector fully.
Note: To replace the Pump tubing (Part No. 29-0240-
12), follow the instructions presented in the ÄKTA start
Maintenance Manual.
1.
2.
3.
4.
5.
6.
A typical System performance test result le:
UNICORN start
System
System control
Performance Test and
Report
Operation from UNICORN start
Instruction
Select method based on the Fraction Collector
conguration: a) Performance method with Frac: when
Fraction collector is enabled; b) Performance method
without Frac: when Fraction collector is disabled.
Read the method notes before starting the run.
Make a note of the result le location.
Run the performance method.
The test report states whether the performance test
passed or failed.
Note: Verify Pressure limits, Fractionation/collection
volumes, gradient levels and all connections for leakage
during the test, using the Acceptance criteria below.
1.
2.
3.
4.
5.
Acceptance criteria
Time
(min)
Activity Check Aproved
interval
1 1 ml/min, 0 %B, ow
through Outlet valve,
Waste position
Back pressure =< 0.05 MPa
2 Repeat UV Auto Zero
3 5 ml/min Back pressure 0.06 - 0.2 MPa
15 Start gradient,
0-100 %B in 10 min,
start fractionation/
collection
19 End fractionation
(with Fraction
collector)
Weigh fraction no
2, 3 and 4
0.8 - 1.2 g
Max di. between
fractions
0.1 g
20
End collection (with-
out Fraction collector)
Weigh beaker 4.2 - 5.8 g
25
End gradient, stay at
100 %B
Gradient (UV 50%
B / UV 100% B)
Straight, no
negative dips.
41 End Check all connec-
tions for leakage
No leakage

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GE akta start Specifications

General IconGeneral
BrandGE
Modelakta start
CategoryLaboratory Equipment
LanguageEnglish

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