ÄKTA start Maintenance Cue Card, 29-0240-43 AC
4
Flow path overview
The illustration below shows the ow path for ÄKTA start.
Part Description Part Description Part Description
1 Buer valve 5 Pressure sensor 9 UV Monitor
2 Mixer 6 Wash valve 10 Conductivity Monitor
3 Sample valve 7 Injection valve (manual) 11 Outlet valve
4 Pump 8 Column 12 Fraction collector
Regular maintenance
Regular maintenance is important for safe and trouble-
free operation of ÄKTA start. The user should perform
maintenance on a daily, weekly and monthly basis.
Periodic maintenance schedule
The periodic maintenance schedule provides a guide to
the user to ensure proper maintenance of the ÄKTA start
and Frac30.
Interval Maintenance action
Daily Visually inspect the instrument for leakages
in the ow path.
If there are signs of liquid leaking, check
integrity of tubing and tubing connections.
Clean the column and ow path after use
and leave the system lled with DM water.
Note: If the instrument is not going to be used
for 1-2 days, prepare the system for storage.
Note: Open the pump cover after switching
o the instrument.
Interval Maintenance action
Weekly,
or when
required
Visually inspect the inlet lters and clean
them if necessary.
Cleaning procedure: immerse and leave the
inlet lters in 1 M NaOH for about 2 hours.
Clean the system ow path with 0.5-1 M
NaOH and rinse with demineralized (DM)
water.
Note: Do not leave the system in NaOH for
more than 20 min.
Note: Cleaning may be necessary more or
less frequently, depending on the system
usage and the nature of the samples.
Calibrate the Pump.
When
required
Clean the instrument externally.
Cleaning procedure: Wipe the surface with
a damp cloth. Wipe o stains using a mild
cleaning agent or 20% ethanol.