GE akta start Maintenance

ÄKTA start Maintenance Cue Card, 29-0240-43 AC
5
Cleaning system ow path
Purpose
The system methods are used for cleaning the system ow
path, including all the inlets and outlets. The following wash
methods are available:
Pump wash A, Pump wash B
Washout fractionation tubing
System cleaning
Pump wash A/B
The Pump wash method is used to wash the selected
inlets/outlets and ow path tubing at high ow rate using
the specied buer port. Pump wash prevents cross-
contamination and carryover of buers/samples.
The Pump wash method is recommended:
Before starting a run when the system is not in use for a
prolonged period of time
When changing buers
Instruction
Immerse the buer A or B inlet tubing in DM water or
buer of choice.
Follow the workow below to run Pump wash.
Operation from the Instrument Display
Perform the following operations to run Pump wash.
•
•
•
•
•
1.
2.
Select
method
Method run
Prepare system
Pump wash A
End
Run
Exit
Pump wash B
Immerse
inlet A
in Buffer A
Immerse
inlet B
in Buffer B
ÄKTA start
Interval Maintenance action
When
required
Clean the Fraction collector.
Note: Frac30 is not a spill-free design. There
will be some spillage when tubes shift
position, depending on the ow rate.
Perform System cleaning, Pump Wash A
and B, Washout Fractionation tubing.
Clean the UV ow cell.
Clean the Conductivity cell.
Calibrate UV.
Calibrate Conductivity.
Perform Zero oset for the Pressure sensor.
Replace the inlet lters.
Replace the tubing and connectors.
Yearly,
or when
required
Check the drive sleeve on the Fraction
collector. Replace if worn out.
Operation from UNICORN start
Perform the following operations to run Pump wash.
UNICORN start
Method run
System control
Pre defined
System preparation
Pump wash A /
Pump wash B
Washout fractionation tubing
The Washout fractionation tubing method is used
to clean the fractionation tubing. The method is
recommended:
Before collecting the fractions using the Outlet valve
without Fraction collector
When changing buers
•
•
Instruction
Immerse the buer inlet tubing in DM water or the
buer of choice.
Disconnect the column from the ow path and
reconnect the tubing with a Union connector.
Follow the workow below to run Washout
fractionation tubing.
1.
2.
3.

GE akta start Specifications

General

Brand	GE
Model	akta start
Category	Laboratory Equipment
Language	English