ÄKTA start Maintenance Cue Card, 29-0240-43 AC 6
Operation from the Instrument Display
Perform the following operations to run Washout
fractionation tubing.
System cleaning
The System cleaning method is used to clean the
instrument ow path. The method is recommended for:
Routine maintenance
Preparing the system for storage
Removing/preventing contamination (bacterial growth)
in the ow path
Preventing carryover of buers/samples
Cleaning solutions
1 M NaOH
DM water
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Instruction
Clean the inlets and outlets (buer tubing, sample inlet
tubing, fractionation tubing)
Immerse the buer inlet tubing (A & B) in 1 M NaOH.
Disconnect column from the ow path and reconnect
the tubing with a Union connector.
Follow the workow below to run System cleaning.
1.
2.
3.
4.
Note:
Do not leave NaOH in the system. Wash the ow path
with water thoroughly.
Check the pH after water wash to ensure complete
removal of NaOH.
It is recommended not to end the method prior to its
completion.
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Operation from the Instrument Display
Method run
Prepare system
Follow the instructions
& tap Continue
Run ended
Exit
System cleaning
When prompted, immerse the
buffer inlets (A & B) in DM water
Continue
ÄKTA start