Invitrogen Qubit 2.0 - Calibrating the Qubit 2.0 Fluorometer; Running New Calibration Standards

14
Calibrating the Qubit
®
2.0 Fluorometer
Introduction
For each assay, you have the choice to run new standards for
calibrating the Qubit
®
2.0 Fluorometer or to use the values from
the previous calibration (for more information, see Calibrating the
Qubit
®
2.0 Fluorometer in the Appendix, page 35). This section
provides instructions for running new calibration standards and
for using the last calibration.
Note: When the fluorometer is used for the first time, there will not be an
option to use the last calibration.
Calibration of the Qubit
®
2.0 Fluorometer requires the preparation
of the appropriate standard solutions. For instructions on the
preparation of these standards, see the instructions that
accompany the assay you are using.
Materials
Needed
 Appropriate standards for the assay you are performing
Note: Incubate the standards for the appropriate amount of time after
mixing them with the working solution (2 minutes for the Qubit
™
DNA and RNA assays, 15 minutes for the Qubit
™
protein assay).
 Qubit
®
assay tubes or other appropriate 0.5 mL assay tubes
 Optional: Qubit
®
2.0 USB drive for data transfer, supplied with
the instrument or available separately
Running New
Standards for
Calibration
1. On the Home Screen, choose the type of assay for which you
want to run the standards. Standards Screen is automatically
displayed.
Continued on next page